Journal article
Escherichia coli Nissle 1917 secondary metabolism: aryl polyene biosynthesis and phosphopantetheinyl transferase crosstalk
Applied microbiology and biotechnology, v 105(20), pp 7785-7799
Oct 2021
PMID: 34546406
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Escherichia coli Nissle 1917 (EcN) is a Gram-negative bacterium that is used to treat inflammatory bowel diseases. The probiotic character of EcN is not well-understood, but its ability to produce secondary metabolites plays an important role in its activity. The EcN genome encodes for an aryl polyene (APE) biosynthetic gene cluster (BGC), and APE products have a role in biofilm formation. We show here that this unusual polyketide assembly line synthase produces four APE molecules which are likely cis/trans isomers. Within the APE BGC, two acyl carrier proteins are involved in biosynthesis. Acyl carrier proteins require activation by post-translational modification with a phosphopantetheinyl transferase (PPTase). Through analysis of single, double, and triple mutants of three PPTases, the PPTase-BGC crosstalk relationship in EcN was characterized. Understanding PPTase-BGC crosstalk is important for the engineering of secondary metabolite production hosts and for targeting of PPTases with new antibiotics. KEY POINTS: • Escherichia coli Nissle 1917 biosynthesizes four aryl polyene isoforms. • Phosphopantetheinyl transferase crosstalk is important for biosynthesis.
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Details
- Title
- Escherichia coli Nissle 1917 secondary metabolism: aryl polyene biosynthesis and phosphopantetheinyl transferase crosstalk
- Creators
- Courtney V Jones - Drexel UniversityBrianna G Jarboe - Drexel UniversityHaley M Majer - Drexel UniversityAmy T Ma - Drexel UniversityJoris Beld - Drexel University
- Publication Details
- Applied microbiology and biotechnology, v 105(20), pp 7785-7799
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000697610200001
- Scopus ID
- 2-s2.0-85115237779
- Other Identifier
- 991019168321904721
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- Web of Science research areas
- Biotechnology & Applied Microbiology