Journal article
Escherichia coli O157:H7 detection limit of millimeter-sized PZT cantilever sensors is 700 cells/mL
Analytical sciences, v 21(4), pp 355-357
Apr 2005
PMID: 15844324
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A composite self-excited millimeter-sized lead zirconate titanate (PZT) glass cantilever (2 mm x 1.8 mm; sensing area of 6 mm2) was fabricated for the detection of Escherichia coli (E. coli) O157:H7. The fundamental and second mode resonance in air was 10.95 +/- 0.05 kHz and 43.45 +/- 0.05 kHz, respectively. Affinity purified monoclonal antibody (anti-E. coli O157:H7) specific to the pathogen E. coli O157:H7 was immobilized at the cantilever glass tip, and then immersed in liquid containing the pathogen (70 to 7 x 10(7) cells/mL). The resonant frequency showed a reduction and reached a steady state shift of 0 +/- 5, 46 +/- 5, 260 +/- 5, and 1010 +/- 5 Hz corresponding to 0, 700, 7000, and 7 x 10(7) cells/mL. From the experiments conducted, the detection limit of the sensor was 700 cells/mL.
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Details
- Title
- Escherichia coli O157:H7 detection limit of millimeter-sized PZT cantilever sensors is 700 cells/mL
- Creators
- Gossett A Campbell - Department of Chemical Engineering, Drexel University, Philadelphia, PA 19104, USARaj Mutharasan
- Publication Details
- Analytical sciences, v 21(4), pp 355-357
- Publisher
- Japan
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000228342900003
- Scopus ID
- 2-s2.0-17744375200
- Other Identifier
- 991014878010204721
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- Web of Science research areas
- Chemistry, Analytical