Journal article
Escherichia coli cellular responses to exposure to atmospheric‐pressure dielectric barrier discharge plasma‐treated N‐acetylcysteine solution
Journal of applied microbiology, v 125(2), pp 383-397
Aug 2018
PMID: 29624820
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Aim
To understand the underlying cellular mechanisms during inactivation of Escherichia coli in response to antimicrobial solution of nonthermal plasma‐activated N‐acetylcysteine (NAC).
Methods and Results
The recommended techniques were used to demonstrate E. coli cellular and transcriptomic changes caused associated with peroxynitrite and compared with plasma‐treated NAC solution. The findings demonstrate that E. coli cells respond to plasma‐treated NAC and undergo severe oxidative and nitrosative stress, and leading to stress‐induced damages to different components of bacterial cells, which includes loss of membrane potential, formation of oxidized glutathione (GSSG), formation of nitrotyrosine (a known marker of nitrosative stress), DNA damage, and generated a prominent pool of peroxynitrite. Reverse‐transcriptase (RT)‐polymerase chain reaction analysis of reactive nitrogen species (RNS) responsive genes indicated their differential expressions.
Conclusion
For the first time, we report that the plasma‐treated NAC solution activates predominantly nitrosative stress‐responsive genes in E. coli and is responsible for cell death.
Significance and Impact of the Study
The reactive species generated in solutions by nonthermal plasma treatment depends on the type of solution or solvent used. The plasma‐treated NAC solution rapidly inactivates E. coli, mostly involving highly RNS generated in NAC solution, and has high potential as disinfectant.
Metrics
Details
- Title
- Escherichia coli cellular responses to exposure to atmospheric‐pressure dielectric barrier discharge plasma‐treated N‐acetylcysteine solution
- Creators
- U.K. Ercan - Drexel UniversityB. Sen - Drexel UniversityA.D. Brooks - Drexel UniversityS.G. Joshi - Drexel University
- Publication Details
- Journal of applied microbiology, v 125(2), pp 383-397
- Publisher
- Wiley
- Number of pages
- 15
- Grant note
- Drexel University College of Medicine Surgical Infection Research Program of the Department of Surgery
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; School of Biomedical Engineering, Science, and Health Systems; Surgery
- Web of Science ID
- WOS:000438344800007
- Scopus ID
- 2-s2.0-85046341829
- Other Identifier
- 991019168162304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology