Journal article
E‐selectin‐mediated dynamic interactions of breast‐and colon‐cancer cells with endothelial‐cell monolayers
International journal of cancer, v 60(3), pp 426-431
27 Jan 1995
PMID: 7530236
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The molecular mechanisms involved in the dynamic interaction of human breast carcinoma cells with the endothelial cell lining of lymphatic vessels and post‐capillary blood venules are largely unknown. In the present study, laminar flow assays were used to investigate the ability of various normal breast cells and of breast‐ and colon‐tumor cells to adhere to human umbilical cord endothelial cell monolayers. MCF‐10A breast, MCF‐7 and T‐47D breast‐carcinoma and clone A, RKO, and HT‐29 colon‐carcinoma cells accumulated and rolled, in the presence of flow, on tumor necrosis factor (TNF)‐stimulated but not on unstimulated endothelial cell monolayers. Non‐tumor and tumor cells continued to form transient adhesions with TNF‐stimulated endothelial cells even when the flow rate was increased to levels found in arteries. Incubation of TNF‐stimulated endothelial cells with an E‐selectin‐specific monoclonal antibody (MAb) partially or completely inhibited dynamic interactions and diminished adhesion strength, whereas integrin β1‐ and integrin α6‐specific MAbs had no effect. A set of highly invasive breast‐carcinoma cells (MDA‐231, BT‐549, HS‐578t) neither adhered to nor rolled on resting or TNF‐stimulated endothelial cell monolayers. However, after 5 min of static incubation, a fraction of these cells attached strongly to resting and TNF‐stimulated endothelial cells and this static adhesion could not be blocked by an E‐selectin‐specific monoclonal antibody. Our results suggest that E‐selectin is a major homing receptor in the metastasis of some breast and colon cancers. © 1995 Wiley‐Liss, Inc.
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Details
- Title
- E‐selectin‐mediated dynamic interactions of breast‐and colon‐cancer cells with endothelial‐cell monolayers
- Creators
- Aydin TözerenHynda K KleinmanDerrick S GrantDavid MoralesArthur M MercurioStephen W Byers
- Publication Details
- International journal of cancer, v 60(3), pp 426-431
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; New York
- Number of pages
- 6
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:A1995QG23500025
- Scopus ID
- 2-s2.0-0028839989
- Other Identifier
- 991014878311604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Oncology