Journal article
Establishment of HIV-1 latency in resting CD4(+) T cells depends on chemokine-induced changes in the actin cytoskeleton
Proceedings of the National Academy of Sciences - PNAS, v 107(39), pp 16934-16939
28 Sep 2010
PMID: 20837531
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Eradication of HIV-1 with highly active antiretroviral therapy (HAART) is not possible due to the persistence of long-lived, latently infected resting memory CD4(+) T cells. We now show that HIV-1 latency can be established in resting CD4(+) T cells infected with HIV-1 after exposure to ligands for CCR7 (CCL19), CXCR3 (CXCL9 and CXCL10), and CCR6 (CCL20) but not in unactivated CD4(+) T cells. The mechanism did not involve cell activation or significant changes in gene expression, but was associated with rapid dephosphorylation of cofilin and changes in filamentous actin. Incubation with chemokine before infection led to efficient HIV-1 nuclear localization and integration and this was inhibited by the actin stabilizer jasplakinolide. We propose a unique pathway for establishment of latency by direct HIV-1 infection of resting CD4(+) T cells during normal chemokine-directed recirculation of CD4(+) T cells between blood and tissue.
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Details
- Title
- Establishment of HIV-1 latency in resting CD4(+) T cells depends on chemokine-induced changes in the actin cytoskeleton
- Creators
- Paul U. Cameron - The Alfred HospitalSuha Saleh - The Alfred HospitalGeorgina Sallmann - The Alfred HospitalAjantha Solomon - The Alfred HospitalFiona Wightman - The Alfred HospitalVanessa A. Evans - The Alfred HospitalGenevieve Boucher - Université de MontréalElias K. Haddad - Université de MontréalRafick-Pierre Sekaly - Université de MontréalAndrew N. Harman - Millennium InstituteJenny L. Anderson - Burnet InstituteKate L. Jones - Burnet InstituteJohnson Mak - Burnet InstituteAnthony L. Cunningham - The University of SydneyAnthony Jaworowski - The Alfred HospitalSharon R. Lewin - The Alfred Hospital
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 107(39), pp 16934-16939
- Publisher
- Natl Acad Sciences
- Number of pages
- 6
- Grant note
- 358399; 491154; 478100 / National Health and Medical Research Council of Australia; National Health and Medical Research Council (NHMRC) of Australia
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Infectious Diseases (and HIV Medicine); Drexel University
- Web of Science ID
- WOS:000282211700038
- Scopus ID
- 2-s2.0-77958485320
- Other Identifier
- 991020100193704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology