Journal article
Estimating the size of laterally phase separated cholesterol domains in model membranes with Förster resonance energy transfer: a simulation study
Colloids and surfaces, B, Biointerfaces, v 33(1)
2004
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this work, we use two vertically-coupled square two-dimensional lattices to simulate membrane bilayers containing a uniform size distribution of cholesterol immiscible domains of a predetermined size distribution. We substitute cholesterols and phospholipids with their fluorescent analogs and calculate the efficiency of energy transfer as a function of acceptor concentration for four membrane configurations. The simulated efficiency of energy transfer as a function of acceptor concentration data is then fit with an analytical FRET model to estimate the domain size, in the same manner in which experimental FRET data is analyzed. The fitted model parameters (domain size and donor partition coefficient) are compared to the simulation inputs to test the applicability of the FRET model to estimating the size of laterally phase separated cholesterol domains. We show that the FRET model yields good size estimates for domains that range between 1 and 25
nm. We also find that the assumed fluorophore configuration in the FRET model leads to a constant under-prediction of these values. Finally, we demonstrate that when two parameters are open to the fit, the FRET model adequately predicts the donor partition coefficient in addition to the domain size.
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Details
- Title
- Estimating the size of laterally phase separated cholesterol domains in model membranes with Förster resonance energy transfer: a simulation study
- Creators
- Gregory M. Troup - Drexel UniversityThomas N. Tulenko - Thomas Jefferson UniversitySum P. Lee - University of WashingtonSteven P. Wrenn - Drexel University
- Publication Details
- Colloids and surfaces, B, Biointerfaces, v 33(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000188512400009
- Scopus ID
- 2-s2.0-0347359169
- Other Identifier
- 991019167554404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Chemistry, Physical
- Materials Science, Biomaterials