Journal article
Evaluating COVID-19 severity prediction and immune dynamics with NULISAseq: Insights from the IMPACC study
The Journal of immunology (1950), Forthcoming
30 Oct 2025
PMID: 41166719
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The National Institutes of Health-funded IMPACC (IMmunoPhenotyping Assessment in a COVID-19 Cohort) evaluated longitudinal clinical and immunological features of human patients hospitalized for COVID-19. This study focuses on comparing the novel NULISAseq assay with the Olink platform using a subset of participants to assess their efficacy in predicting COVID-19 severity and understanding immune response dynamics. Our findings reveal that NULISAseq could provide superior detectability and dynamic range across various targets. Elastic net analysis demonstrated that specific proteins, including amphiregulin, effectively predict COVID-19 severity from sera at admission (samples drawn within 96 h of admission), with a test area under the curve of 0.84. Longitudinal analysis identified significant differences in multiple targets, including IL-5 and interferons, between low- and high-severity groups over time. Additionally, association rule mining suggested potential early markers predictive of later immune cell changes. These findings emphasize the potential of NULISAseq for comprehensive profiling, early prediction, and identification of targeted therapeutic interventions in COVID-19.
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Details
- Title
- Evaluating COVID-19 severity prediction and immune dynamics with NULISAseq: Insights from the IMPACC study
- Creators
- Koji Abe (Corresponding Author) - Stanford University School of MedicineTyson H Holmes - Stanford University School of MedicineTran T Nguyen - Stanford University School of MedicineSeunghee Kim-Schulze - Icahn School of Medicine at Mount SinaiOfer Levy - Boston Children's HospitalLindsey R Baden - Brigham and Women's HospitalEsther Melamed - The University of Texas at AustinLauren I R Ehrlich - The University of Texas at AustinGrace A McComsey - Case Western Reserve UniversityRafick P Sekaly - Case Western Reserve UniversityCharles B Cairns - Drexel UniversityElias K Haddad - Drexel UniversityAlbert C Shaw - Yale School of MedicineDavid A Hafler - Yale School of MedicineRuth R Montgomery - Yale School of MedicineDavid B Corry - Baylor College of MedicineFarrah Kheradmand - Baylor College of MedicineMark A Atkinson - University of FloridaScott C Brakenridge - University of FloridaNelson I Agudelo Higuita - OU HealthJordan P Metcalf - OU HealthCatherine L Hough - Oregon Health & Science UniversityWilliam B Messer - Oregon Health & Science UniversityBali Pulendran - Stanford University School of MedicineKari C Nadeau - Stanford University School of MedicineMark M Davis - Stanford University School of MedicineAna Fernandez-Sesma - Icahn School of Medicine at Mount SinaiViviana Simon - Icahn School of Medicine at Mount SinaiMonica Kraft - University of ArizonaChris Bime - University of ArizonaCarolyn S Calfee - University of California, San FranciscoDavid J Erle - University of California, San FranciscoJoanna Schaenman - University of California, Los AngelesElaine F Reed - University of California, Los AngelesAl Ozonoff - Boston Children's HospitalBjoern Peters - La Jolla Institute for ImmunologySteven H Kleinstein - Yale School of MedicineAlison D Augustine - National Institute of Allergy and Infectious DiseasesJoann Diray-Arce - Boston Children's HospitalPatrice M Becker - National Institute of Allergy and Infectious DiseasesNadine Rouphael - Emory and Henry CollegeHolden T Maecker - Stanford University School of MedicineIMPACC Network
- Publication Details
- The Journal of immunology (1950), Forthcoming
- Publisher
- Oxford University Press
- Number of pages
- 11
- Grant note
- 5U19AI057229-18 / NIH HHS 5R01AI135803-03 / NIH HHS 5U54AI142766-03 / NIH HHS 3U19AI1289130 / NIH HHS 3U01AI167892-03S2 / NIH HHS 5U19AI128913-03 / NIH HHS 3U19AI128913-03 / NIH HHS 5U19AI057229-17 / NIH HHS National Institute of Allergy and Infectious Diseases 5R01AI104870-07 / NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine; Infectious Diseases (and HIV Medicine); Emergency Medicine
- Web of Science ID
- WOS:001605950200001
- Scopus ID
- 2-s2.0-105025824927
- Other Identifier
- 991022129387404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology