Journal article
Evaluation of Cellular and Systemic Toxicity of Dielectric Barrier Discharge Plasma-Treated N-Acetylcysteine as Potential Antimicrobial Catheter Lock Solution
Plasma, v 4(4), pp 732-744
01 Dec 2021
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Intravenous catheter-related bloodstream infections are a cause of remarkable problems. Catheter lock solutions are used to keep catheter patency and prevent catheter-related bloodstream infections. The leakage of catheter lock solution to the bloodstream raises toxicity concerns. Plasma-treated liquids carry the potential to act as catheter lock solutions by virtue of their strong antimicrobial effects. The present study investigates the tolerance of the proposed solution in vitro and in vivo. N-acetylcysteine (NAC) solution was treated with atmospheric-air DBD plasma and antimicrobial assays were performed. The cytotoxicity of the plasma-treated NAC solution was evaluated on an EA.hy926 cell line. Intravenous administration of plasma-treated NAC solution in different doses was given to Sprague Dawley rats. One week after infusion with plasma-treated NAC solution, first, the blood samples were collected, and then liver, kidney, tail vein, heart, and lung tissue samples were collected from euthanized rats for histopathological examination. The cytotoxicity of plasma-treated NAC solution depended on plasma treatment time, contact time, and cell number. A strong antimicrobial effect with no cytotoxicity of plasma-treated NAC solution was observed in endothelial cells. Based on blood tests and histopathological examination, no signs of systemic toxicity were observed that can be correlated to the plasma-treated-NAC solution. This solution has the potential to be used as a catheter lock solution with strong antimicrobial properties, keeping catheter patency.
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Details
- Title
- Evaluation of Cellular and Systemic Toxicity of Dielectric Barrier Discharge Plasma-Treated N-Acetylcysteine as Potential Antimicrobial Catheter Lock Solution
- Creators
- Utku K. K. Ercan - Izmir Kâtip Çelebi UniversityAdam D. D. Yost - Drexel Univ, Sch Biomed Engn, Sci & Hlth Syst, Philadelphia, PA 19104 USAKimberly Wasko - Drexel UniversityAri D. D. Brooks - Drexel UniversitySuresh G. G. Joshi - Drexel University
- Publication Details
- Plasma, v 4(4), pp 732-744
- Publisher
- Mdpi
- Number of pages
- 13
- Grant note
- intramural funding of the research division of Department of Surgery of Drexel University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Surgery
- Web of Science ID
- WOS:000968856400001
- Scopus ID
- 2-s2.0-85126258059
- Other Identifier
- 991021463546304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Physics, Fluids & Plasmas