Journal article
Evaluation of PfHRP2 and PfLDH Malaria Rapid Diagnostic Test Performance in Assosa Zone, Ethiopia
The American journal of tropical medicine and hygiene, v 103(5), pp 1902-1909
01 Nov 2020
PMID: 32840197
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In malaria-endemic countries, rapid diagnostic tests (RDTs) targeting Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and lactate dehydrogenase (PfLDH) have been widely used. However, little is known regarding the diagnostic performances of these RDTs in the Assosa zone of northwest Ethiopia. The objective of this study was to determine the diagnostic performances of PfHRP2 and PfLDH RDTs using microscopy and quantitative PCR (qPCR) as a reference test. A health facility-based cross-sectional study design was conducted from malaria-suspected study participants at selected health centers from November to December 2018. Finger-prick blood samples were collected for microscopy, RDTs, and qPCR method. The prevalence of P. falciparum was 26.4%, 30.3%, and 24.1% as determined by microscopy, PfHRP2 RDT, and PfLDH RDT, respectively. Compared with microscopy, the sensitivity and specificity of the PfHRP2 RDT were96% and 93%, respectively, and those of the PfLDH RDT were89% and 99%, respectively. Compared with qPCR, the specificity of the PfHRP2 RDT (93%) and PfLDH RDT (98%) was high, but the sensitivity of the PfHRP2 RDT (77%) and PfLDH RDT (70%) was relatively low. These malaria RDTs and reference microscopy methods showed reasonable agreement with a kappa value above 0.85 and provided accurate diagnosis of P. falciparum malaria. Thus, the current malaria RDT in the Ministry of Health program can be used in the Assosa zone of Ethiopia. However, continuous monitoring of the performance of PfHRP2 RDT is important to support control and elimination of malaria in Ethiopia.
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Details
- Title
- Evaluation of PfHRP2 and PfLDH Malaria Rapid Diagnostic Test Performance in Assosa Zone, Ethiopia
- Creators
- Gezahegn Solomon Alemayehu - Addis Ababa UniversityKaren Lopez - University of North Carolina at CharlotteCheikh Cambel Dieng - University of North Carolina at CharlotteEugenia Lo - University of North Carolina at CharlotteDaniel Janies - University of North Carolina at CharlotteLemu Golassa - Addis Ababa University
- Publication Details
- The American journal of tropical medicine and hygiene, v 103(5), pp 1902-1909
- Publisher
- The American Journal of Tropical Medicine and Hygiene
- Number of pages
- 8
- Grant note
- University of NorthCarolina atCharlotte (Department ofBioinformatics andGenomics, College of Computing and Informatics) University of NorthCarolina atCharlotte (Bioinformatics Research Center) NIH R15 AI138002 / National Institute of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA RD/LT/505/2016 / Addis Ababa University thematic grant University of NorthCarolina atCharlotte (Department of Biological Sciences) Belk Family
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000588422900030
- Scopus ID
- 2-s2.0-85095861611
- Other Identifier
- 991022192520504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Public, Environmental & Occupational Health
- Tropical Medicine