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Evaluation of Polymorphic Locus Sequence Typing for Candida glabrata Epidemiology
Journal article   Open access   Peer reviewed

Evaluation of Polymorphic Locus Sequence Typing for Candida glabrata Epidemiology

Santosh Katiyar, Eric Shiffrin, Celeste Shelton, Kelley Healey, John-Paul Vermitsky and Tom Edlind
Journal of clinical microbiology, v 54(4), pp 1042-1050
Apr 2016
DOI: https://doi.org/10.1128/JCM.03106-15
PMID: 26842706
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1128/jcm.03106-15View
Published, Version of Record (VoR),  Open
url
https://doi.org/10.1128/JCM.03106-15View
Published, Version of Record (VoR) Open

Abstract

Candida glabrata - classification Candida glabrata - genetics Candida glabrata - isolation & purification Candidiasis - epidemiology Candidiasis - microbiology Cluster Analysis Genetic Loci Genetic Variation Genotype Humans Molecular Epidemiology - methods Multilocus Sequence Typing - methods Mycological Typing Techniques - methods
The opportunistic yeast Candida glabratais increasingly refractory to antifungal treatment or prophylaxis and relatedly is increasingly implicated in health care-associated infections. To elucidate the epidemiology of these infections, strain typing is required. Sequence-based typing provides multiple advantages over length-based methods, such as pulsed-field gel electrophoresis (PFGE); however, conventional multilocus sequence typing (targeting 6 conserved loci) and whole-genome sequencing are impractical for routine use. A commercial sequence-based typing service for C. glabratathat targets polymorphic tandem repeat-containing loci has recently been developed. These CgMT-J and CgMT-M services were evaluated with 56 epidemiologically unrelated isolates, 4 to 7 fluconazole-susceptible or fluconazole-resistant isolates from each of 5 center A patients, 5 matched pairs of fluconazole-susceptible/resistant isolates from center B patients, and 7 isolates from a center C patient who responded to then failed caspofungin therapy. CgMT-J and CgMT-M generated congruent results, resolving isolates into 24 and 20 alleles, respectively. Isolates from all but one of the center A patients shared the same otherwise rare alleles, suggesting nosocomial transmission. Unexpectedly, Pdr1 sequencing showed that resistance arose independently in each patient. Similarly, most isolates from center B also clustered together; however, this may reflect a dominant clone since their alleles were shared by multiple unrelated isolates. Although distinguishable by their echinocandin susceptibilities, all isolates from the center C patient shared alleles, in agreement with the previously reported relatedness of these isolates based on PFGE. Finally, we show how phylogenetic clusters can be used to provide surrogate parents to analyze the mutational basis for antifungal resistance.

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16 citations in Scopus

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Collaboration types
Domestic collaboration
Web of Science research areas
Microbiology
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