Journal article
Evaluation of core–shell particle columns for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides
Journal of pharmaceutical and biomedical analysis, v 72
18 Jan 2013
PMID: 23146223
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
► Oligonucleotide analysis by ion-pair reversed-phase liquid chromatography. ► Evaluation of different fused-core columns for oligonucleotide analysis. ► Fused-core columns provided improved separations of oligonucleotides. ► The sub-2μm particle fused-core columns showed the best separations. ► Long term column stability was tested for fused-core columns.
An investigation into the use of core–shell particle columns for separation of short (∼21 base pairs) RNA oligonucleotides by ion-pair reversed-phase liquid chromatography (IP-RPLC) showed improved resolution for a number of test analytes relative to conventional (fully-porous) reversed-phase columns. The best resolutions were obtained using columns packed with smaller sub-2μm core–shell particles.
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Details
- Title
- Evaluation of core–shell particle columns for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides
- Creators
- Mirlinda Biba - Merck, Rahway, NJ 07065, USAChristopher J Welch - Merck, Rahway, NJ 07065, USAJoe P Foley - Drexel University, Philadelphia, PA 19101, USABing Mao - Merck, Rahway, NJ 07065, USAEnrique Vazquez - Merck, Rahway, NJ 07065, USARebecca A Arvary - Merck, Rahway, NJ 07065, USA
- Publication Details
- Journal of pharmaceutical and biomedical analysis, v 72
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000311819100004
- Scopus ID
- 2-s2.0-84869075138
- Other Identifier
- 991014878120604721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical
- Pharmacology & Pharmacy