Journal article
Evaluation of new-generation serologic tests for the diagnosis of typhoid fever: data from a community-based surveillance in Calcutta, India
Diagnostic microbiology and infectious disease, v 56(4), pp 359-365
01 Dec 2006
PMID: 16938421
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Although typhoid fever is confirmed by culture of
Salmonella enterica serotype Typhi, rapid and simple diagnostic serologic tests would be useful in developing countries. We examined the performance of Widal test in a community field site and compared it with Typhidot and Tubex tests for diagnosis of typhoid fever. Blood samples were collected from 6697 patients with fever for ≥3 days for microscopy, culture, and serologic testing and from randomly selected 172 consenting healthy individuals to assess the baseline Widal anti-Typhi O lipopolysaccharide antibody (anti-TO) and anti-Typhi H flagellar antibody (anti-TH) titers. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the 3 serologic tests were calculated using culture-confirmed typhoid fever cases as “true positives” and paratyphoid fever and malaria cases as “true negatives”. Comparing cutoff values for the Widal test, an anti-TO titer of 1/80 was optimal with 58% sensitivity, 85% specificity, 69% PPV, and 77% NPV. Sensitivity was increased to 67% when the Widal test was done on the 5th day of illness and thereafter. The sensitivity, specificity, PPV, and NPV of Typhidot and Tubex were not better than Widal test. There is a need for more efficient rapid diagnostic test for typhoid fever especially during the acute stage of the disease. Until then, culture remains the method of choice.
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Details
- Title
- Evaluation of new-generation serologic tests for the diagnosis of typhoid fever: data from a community-based surveillance in Calcutta, India
- Creators
- Shanta Dutta - National Institute of Cholera and Enteric DiseasesDipika Sur - National Institute of Cholera and Enteric DiseasesByomkesh Manna - National Institute of Cholera and Enteric DiseasesBhaswati Sen - National Institute of Cholera and Enteric DiseasesAlok Kumar Deb - National Institute of Cholera and Enteric DiseasesJacqueline L. Deen - International Vaccine InstituteJohn Wain - Wellcome Sanger InstituteLorenz Von Seidlein - International Vaccine InstituteLeon Ochiai - International Vaccine InstituteJohn D. Clemens - International Vaccine InstituteSujit Kumar Bhattacharya - National Institute of Cholera and Enteric Diseases
- Publication Details
- Diagnostic microbiology and infectious disease, v 56(4), pp 359-365
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; College of Medicine
- Web of Science ID
- WOS:000243059700003
- Scopus ID
- 2-s2.0-33845316135
- Other Identifier
- 991021448023404721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Infectious Diseases
- Microbiology