Journal article
Evidence endogenous interferon production contributed to the lack of ocular virulence of an HSV intertypic recombinant
Current eye research, v 10(s1), pp 39-45
1991
PMID: 1650672
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
An intertypic recombinant isolated from rabbit kidney cells following co-transfection of HSV-1(17) and HSV-2(186) DNA failed to induce overt ocular pathology when inoculated onto the murine scarified cornea at concentrations as high as 107 PFU per eye. In contrast, both parents induced corneal disease at a 1000-fold lower dose. The reason (s) for the failure of the intertypic recombinant, designated R025X, to induce corneal pathology was investigated. It was found that the recombinant was 100-fold more sensitive to the inhibitory effects of interferon (FN) α/β than the parent strains in corneal button growth studies in vitro. R025X readily grew in cultured mouse corneal fibroblasts at a low multiplicity of infection. However, the peak titer was approximately 8-fold lower than that of strain 17. Addition of rabbit anti-IFN α/β to the culture medium resulted in a 4 to 5-fold increase in infectious titer compared to its growth in the absence of antiserum. Most significantly, when mice were pre-treated in vivo with anti-IFN α/β 24 hours prior to virus corneal infection, 67% of the recipients developed moderate to severe stromal keratitis, whereas none of the controls developed corneal pathology. Blepharitis was also significantly increased in incidence and severity in the antiserum treated hosts. We conclude that the inability of R025X to induce ocular disease was due, at least in part, to the inhibitory effects of interferon produced in response to infection.
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Details
- Title
- Evidence endogenous interferon production contributed to the lack of ocular virulence of an HSV intertypic recombinant
- Creators
- Robert N. Lausch - University of South AlabamaYing-Hsiu Su - University of South AlabamaMary Ritchie - University of South AlabamaJohn E. Oakes - University of South Alabama
- Publication Details
- Current eye research, v 10(s1), pp 39-45
- Publisher
- Informa UK Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1991FM96500006
- Scopus ID
- 2-s2.0-0025905543
- Other Identifier
- 991021463548404721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Ophthalmology