Journal article
Evidence for an association of a ribonucleic acid with the purified, unactivated glucocorticoid receptor
Biochemical and biophysical research communications, v 140(1), pp 204-211
15 Oct 1986
PMID: 2430563
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The unactivated glucocorticoid-receptor complex (GRC) was purified from rat hepatic cytosol (approximately 4000-fold by specific activity) by a procedure developed in our laboratory. Following elution of unactivated GRC from DEAE-cellulose with a 0.05–0.5 M potassium phosphate gradient, a second gradient of 0.5–1.0 M potassium chloride was started. This gradient eluted material at 0.6 M potassium chloride that incorporated
[
32P]
in vivo
and stained with ethidium bromide. A predominant ethidium bromide stained band of 100–110 nucleotides was observed. The presence of this material was dependent on the presence of highly purified GRC since presaturation of cytosol with 50 μM unlabeled triamcinolone acetonide (TA) precluded the appearance of this material. Experiments with partially purified GRC from CEM-C7 cells incubated
in vivo
with [
14C]uridine indicated that the material eluted at 0.6 M potassium chloride incorporated [
14C]-uridine. Collectively, these data suggest that a RNA is associated with the purified, unactivated form of the GRC.
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Details
- Title
- Evidence for an association of a ribonucleic acid with the purified, unactivated glucocorticoid receptor
- Creators
- Maria L. Webb - Temple UniversityThomas J. Schmidt - Temple UniversityNoreen M. Robertson - Temple UniversityGerald Litwack - Temple University
- Publication Details
- Biochemical and biophysical research communications, v 140(1), pp 204-211
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- SOM Dean - Research Administration
- Web of Science ID
- WOS:A1986E443000028
- Scopus ID
- 2-s2.0-0022966131
- Other Identifier
- 991021463561504721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics