Journal article
Evidence for involvement of NFBP in processing of ribosomal RNA
Journal of cellular physiology, v 214(2), pp 381-388
01 Feb 2008
PMID: 17654514
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Ribosomal RNA (rRNA) in vertebrates is initially transcribed as a single 47S precursor which is modified by the addition of 2'-O-methyl ribose moieties, pseudouridines, and methyl groups, followed by cleavage at several sites to produce the mature 28S, 18S, and 5.8S rRNAs. Cleavage of the rRNA precursor to generate the 18S rRNA is mediated by a ribonucleoprotein (RNP) complex termed the processome containing U3, a box C/D small nucleolar RNA (snoRNA), and at least 28 cellular proteins. We previously identified a novel human RNA binding protein, NF-kappa B binding protein (NFBP), which is the human homolog of Rrp5p, a protein component of the yeast U3 processome. Here, we show that NFBP colocalizes with and coprecipitates U3 in the nucleolus. We also demonstrate that NFBP is essential for the generation of 18S rRNA as maturation of the 18S rRNA is repressed in the absence of NFBP. Using Northern blot analyses, we further show that NFBP is specifically necessary for cleavages at sites A0, 1, and 2, as unprocessed intermediate forms of rRNA accumulated in the absence of NFBP.
Metrics
Details
- Title
- Evidence for involvement of NFBP in processing of ribosomal RNA
- Creators
- Thersa Sweet - Ctr Neurovirol, Dept Neurosci, Philadelphia, PA 19122 USAWilliam Yen - Ctr Neurovirol, Dept Neurosci, Philadelphia, PA 19122 USAKamel Khalili - Ctr Neurovirol, Dept Neurosci, Philadelphia, PA 19122 USAShohreh Amini - Ctr Neurovirol, Dept Neurosci, Philadelphia, PA 19122 USA
- Publication Details
- Journal of cellular physiology, v 214(2), pp 381-388
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- K01MH069128 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) KO1 MH069128. / NIMH NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Epidemiology and Biostatistics
- Web of Science ID
- WOS:000252163400012
- Scopus ID
- 2-s2.0-37349131975
- Other Identifier
- 991021448053504721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology
- Physiology