Evidence for involvement of NFBP in processing of rRNA

T Sweet; W Yen; K Khalili; S Amini

NF-kappaB Binding Protein (NFBP) is an RNA binding protein identified due to its ability to bind to NF-kappaB subunits, p50 and p65. NF-kappa B proteins are a family of inducible transcription factors which activate many cellular genes involved in apoptosis, the immune response and cellular proliferation. Additionally, viruses such as HIV-1 utilize NF-kappaB to activate viral transcription. Our previous work demonstrated that NFBP is involved in activation of HIV-1 LTR via its interactions with the cellular NF-kappaB complex and with the viral protein, tat. In order to investigate the role of NFBP in a normal cellular environment, we searched for homologous proteins from other species and found that NFBP shares 24% amino acid similarity to RRP5, a protein involved in processing of ribosomal RNA (rRNA) in yeast. In humans, rRNA is initially transcribed as a single 47S precursor which is modified by the addition of 2'-O-methyl ribose moieties, pseudouridines, and methyl groups, followed by cleavage at several sites to produce the mature 28S, 18S and 5.8S rRNAs. Cleavage of the rRNA precursor to generate the 18S rRNA is mediated by a ribonucleoprotein (RNP) complex termed the processome containing U3, a box C/D snoRNA, and at least 28 cellular proteins. We detected endogenous NFBP in the nucleolus, where it colocalizes with and binds to the U3 snoRNA. Furthermore, by employing siRNA techniques to target NFBP, we demonstrate that NFBP is essential for several early cleavage events necessary for maturation of 18S rRNA. Our results demonstrate that NFBP is a vital component of rRNA processing in human cells.

Evidence for involvement of NFBP in processing of rRNA

Additional Links

Abstract

Metrics

Details

Evidence for involvement of NFBP in processing of rRNA

Additional Links

Abstract

Metrics

Details

Drexel University Social media