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Evidence for prostate cancer-associated diagnostic marker-1: immunohistochemistry and in situ hybridization studies
Journal article   Open access   Peer reviewed

Evidence for prostate cancer-associated diagnostic marker-1: immunohistochemistry and in situ hybridization studies

Akira Ohkia, Youji Hu, Min Wang, Fernando U Garcia and Mark E Stearns
Clinical cancer research, v 10(7), pp 2452-2458
01 Apr 2004
DOI: https://doi.org/10.1158/1078-0432.CCR-03-0170
PMID: 15073124
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1158/1078-0432.ccr-03-0170View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze),  Open
url
https://doi.org/10.1158/1078-0432.CCR-03-0170View
Published, Version of Record (VoR) Open

Abstract

Antigens, Neoplasm - biosynthesis Antigens, Neoplasm - genetics Biomarkers, Tumor Blotting, Western Cell Line Cell Line, Tumor Humans Hyperplasia Immunohistochemistry In Situ Hybridization Male Prostate - metabolism Prostatic Intraepithelial Neoplasia - pathology Prostatic Neoplasms - diagnosis Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Recombinant Proteins - chemistry RNA, Messenger - metabolism Software
The purpose of this study was to characterize a novel gene/protein associated with prostate cancer, termed prostate cancer-associated diagnostic marker-1 [PCADM-1 (Hu Y, Wang M, Garcia FU, Aoyaki K, Stearns ME. Identification of PCADM-1 as a novel diagnostic marker for prostate cancer, submitted for publication)]. Immunological studies revealed that rabbit polyclonal antibodies generated against recombinant PCADM-1 specifically recognize the protein in crude protein extracts from a variety of prostate cancer cell lines (i.e., PC-3 ML, LNCaP, DU145, and CPTX-1532) and prostate cancer tissue. Combined immunolabeling and in situ hybridization studies demonstrated that PCADM-1 mRNA was expressed by the luminal epithelial cells of prostate cancer glands and was not expressed by high-grade prostatic intraepithelial neoplasia or HPV-MLC7 cells. Immunolabeling studies of tissue arrays from biopsies of archival material (n = 200 samples) confirmed that PCADM-1 was expressed by the luminal epithelial cells of prostate cancer. Taken together, the data suggest that PCADM-1 is a specific marker for human prostate cancer.

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10 citations in Web of Science
13 citations in Scopus

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Oncology
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