Journal article
Evidence that oocyte maturation induced by an oncogenic ras-p21 protein and insulin is mediated by overlapping yet distinct mechanisms
Experimental cell research, v 203(2), pp 329-335
1992
PMID: 1459198
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have recently shown that a peptide (residues 35–47) from a functional region of the
ras p21 protein, thought to be involved in the binding of p21 to GTPase activating protein, the antibiotic azatyrosine, known to induce the
ras-recision gene, and the selective protein kinase C inhibitor, CGP 41 251, all inhibit oncogenic p21 protein-induced maturation of oocytes in a dose-dependent manner. We now show that these three agents only partially inhibit insulin-induced oocyte maturation, known to be dependent on activation of cellular p21 protein. On the other hand, the anti-p21 protein antibody Y13–259 completely inhibits both insulin- and oncogenic p21 protein-induced maturation as does a tetrapeptide, CVIM, known to block the enzyme farnesyl transferase which covalently attaches the farnesyl moiety to the p21 protein allowing it to attach to the cell membrane. Our results suggest that while the oncogenic and insulin-activated normal p21 proteins share certain elements of their signal transduction pathways in common, these pathways diverge and allow for selective inhibition of the oncogenic pathway.
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Details
- Title
- Evidence that oocyte maturation induced by an oncogenic ras-p21 protein and insulin is mediated by overlapping yet distinct mechanisms
- Creators
- Denise L. Chung - New York UniversityAlvin Joran - New York UniversityFred Friedman - National Institutes of HealthRichard Robinson - National Institutes of HealthPaul W. Brandt-Rauf - Columbia UniversityI.Bernard Weinstein - Columbia UniversityZeev Ronai - American Health Foundation, Valhalla, New York 10595 U.S.ALeonard Baskin - Institute for Cancer PreventionDaryll C. Dykes - Syracuse UniversityRandall B. Murphy - New York UniversitySusumu Nishimura - National Cancer Research InstituteZ. Yamaizumi - National Cancer Research InstituteMatthew R. Pincus - Syracuse University
- Publication Details
- Experimental cell research, v 203(2), pp 329-335
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:A1992KD84200006
- Scopus ID
- 2-s2.0-13644255338
- Other Identifier
- 991019342553404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Oncology