Journal article
Evidence that the immediate early gene product, ICP4, is necessary for HSV-1 DNA circularization in infected cells
Journal of neurovirology, Vol.12, pp.78-78
01 May 2006
Abstract
In this study, we demonstrated that the ICP4 protein is involved in the process of the endless formation of infecting linear HSV-1 genome through the following evidences: (1) The ICP4 mutant virus d120 genome failed to form the endless conformation when it infected in Vero cells, whereas, a significant portion (approximate 50%) of the genomes of wild-type strain KOS formed the endless conformation after infection on Vero cells in the presence of 400 mu g/ml of phosphonacetic acid (PAA) which is a DNA replication inhibitor,; (2) A significant portion (approximate 60%) of the genomes of ICP4 mutant d120 formed the endless conformation after infection in its complementary cells (E5), which expressed the ICP4 protein after d120 mutant virus infection; (3) When ICP4 proteins were provided by a DNA replication defected DNA polymerase mutant HP66, the genomes of mutant d120 could form the endless conformation in Vero cells. Western analysis using antibody specific to the ICP4 protein shows that although the d120 virions, purified from the d120-infected E5 cells, contained ICP4 proteins, the majority of ICP4 proteins associated with the d120 virions are in the 40 KD truncated form with a small fraction of the full-length 175 KD ICP4 protein. These d120 virion associated ICP4 proteins were unable to mediate the endless formation after infection in E5 cells when the expression of the ICP4 protein from E5 cells was inhibited by 200 mu g/ml of cycloheximide. Collectively, this data suggest that the ICP4 protein has an important, previously unreported, role in mediating the endless formation of genome upon infection and that this function can be provided in trans.
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Details
- Title
- Evidence that the immediate early gene product, ICP4, is necessary for HSV-1 DNA circularization in infected cells
- Creators
- Ying-Hsiu SuXianchao ZhangN FraserT Block
- Publication Details
- Journal of neurovirology, Vol.12, pp.78-78
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170150904721