Files and links (1)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Evolution of acyl-ACP thioesterases and beta-ketoacyl-ACP synthases revealed by protein-protein interactions
Journal of applied phycology, v 26(4), pp 1619-1629
01 Aug 2014
PMID: 25110394
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The fatty acid synthase (FAS) is a conserved primary metabolic enzyme complex capable of tolerating cross-species engineering of domains for the development of modified and overproduced fatty acids. In eukaryotes, acyl-acyl carrier protein thioesterases (TEs) off-load mature cargo from the acyl carrier protein (ACP), and plants have developed TEs for short/medium-chain fatty acids. We showed that engineering plant TEs into the green microalga Chlamydomonas reinhardtii does not result in the predicted shift in fatty acid profile. Since fatty acid biosynthesis relies on substrate recognition and protein-protein interactions between the ACP and its partner enzymes, we hypothesized that plant TEs and algal ACP do not functionally interact. Phylogenetic analysis revealed major evolutionary differences between FAS enzymes, including TEs and ketoacyl synthases (KSs), in which the former is present only in some species, whereas the latter is present in all, and has a common ancestor. In line with these results, TEs appeared to be selective towards their ACP partners, whereas KSs showed promiscuous behavior across bacterial, plant, and algal species. Based on phylogenetic analyses, in silico docking, in vitro mechanistic cross-linking, and in vivo algal engineering, we propose that phylogeny can predict effective interactions between ACPs and partner enzymes.
Metrics
Details
- Title
- Evolution of acyl-ACP thioesterases and beta-ketoacyl-ACP synthases revealed by protein-protein interactions
- Creators
- Joris Beld - University of California San DiegoJillian L. Blatti - Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USACraig Behnke - Sapphire Energy Inc, San Diego, CA USAMichael Mendez - Sapphire Energy Inc, San Diego, CA USAMichael D. Burkart - Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA
- Publication Details
- Journal of applied phycology, v 26(4), pp 1619-1629
- Publisher
- Springer Nature
- Number of pages
- 11
- Grant note
- DE-EE0003373 / Department of Energy; United States Department of Energy (DOE) 500-10-039 / California Energy Commission CILMSF R01GM094924; R01GM095970 / National Institute of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000340101100001
- Scopus ID
- 2-s2.0-84905264609
- Other Identifier
- 991020535054004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Marine & Freshwater Biology