Journal article
Ex Vivo Live Imaging in Brain Slices for Analysis of Neurite Formation in Combination with In Utero Electroporation
Methods in molecular biology (Clifton, N.J.), v 2831, pp 199-208
2024
PMID: 39134851
Abstract
The use of time-lapse live imaging enables us to track the dynamic changes in neurites during their formation. Ex vivo live imaging with acute brain slices provides a more physiological environment than cultured cells. To accomplish this, a certain method of labeling is necessary to visualize and identify neurite morphology. To understand the dynamics of neurite structure at early stages of neurite formation, we describe in this chapter ex vivo live imaging using a confocal microscope at P0 in combination with in utero electroporation (IUE).
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Details
- Title
- Ex Vivo Live Imaging in Brain Slices for Analysis of Neurite Formation in Combination with In Utero Electroporation
- Creators
- Xiaonan Liu - Drexel UniversityKazuhito Toyo-Oka (Corresponding Author) - Drexel University
- Publication Details
- Methods in molecular biology (Clifton, N.J.), v 2831, pp 199-208
- Publisher
- Springer Nature
- Grant note
- R01 NS096098 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Scopus ID
- 2-s2.0-85201253167
- Other Identifier
- 9781071639696; 1071639692; 991022202494504721