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Exercise Reduces Age-dependent Decrease in Platelet Protein Kinase C Activity and Translocation
Journal article   Peer reviewed

Exercise Reduces Age-dependent Decrease in Platelet Protein Kinase C Activity and Translocation

Hoau-Yan Wang, Theodore R. Bashore and Eitan Friedman
The journals of gerontology. Series A, Biological sciences and medical sciences, v 50A(1), pp M12-M16
Jan 1995
PMID: 7814783

Abstract

Background. This study examined the effect of age and aerobic exercise performed on a regular basis on human platelet protein kinase C (PKC) activity and translocation. Methods. Blood platelets were obtained from young (20-36 years) and older (61-78 years) healthy male human subjects. Platelets were incubated with PMA, 5-HT, or thrombin; PKC activities were measured in partially purified extracts of cytosolic and membranous fractions. Results. Platelet PKC activities associated with both the membranes and the cytosol cellular fractions were significantly reduced in older nonexercisers. Redistribution of platelet PKC activity elicited by stimulating the cell surface receptors for 5-HT or thrombin or by direct PKC stimulation (PMA) was found to be reduced in the elderly subjects. Maintenance of aerobic fitness in the older group of exercisers partially prevented the age-associated decline in platelet PKC activity and in stimuli-induced enzyme redistribution. Conclusions. These results indicate that platelet PKC activity and its translocation may be biological markers of aging and that aerobic exercise may serve to slow the rate at which enzyme activation declines during senescence.

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Collaboration types
Domestic collaboration
Web of Science research areas
Geriatrics & Gerontology
Gerontology
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