Journal article
Exploring Biomolecular Recognition Using Optical Biosensors
Methods (San Diego, Calif.), v 19(2), pp 253-269
Oct 1999
PMID: 10527730
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Understanding the basic forces that determine molecular recognition helps to elucidate mechanisms of biological processes and facilitates discovery of innovative biotechnological methods and materials for therapeutics, diagnostics, and separation science. The ability to measure interaction properties of biological macromolecules quantitatively across a wide range of affinity, size, and purity is a growing need of studies aimed at characterizing biomolecular interactions and the structural elements that drive them. Optical biosensors have provided an increasingly impactful technology for such biomolecular interaction analyses. These biosensors record the binding and dissociation of macromolecules in real time by transducing the accumulation of mass of an analyte molecule at the sensor surface coated with ligand molecule into an optical signal. Interactions of analytes and ligands can be analyzed at a microscale and without the need to label either interactant. Sensors enable the detection of bimolecular interaction as well as multimolecular assembly. Most notably, the method is quantitative and kinetic, enabling determination of both steady-state and dynamic parameters of interaction. This article describes the basic methodology of optical biosensors and presents several examples of its use to investigate such biomolecular systems as cytokine growth factor–receptor recognition, coagulation factor assembly, and virus–cell docking.
Metrics
Details
- Title
- Exploring Biomolecular Recognition Using Optical Biosensors
- Creators
- Gabriela Canziani - Department of Medicine, School of MedicineWentao Zhang - Department of Medicine, School of MedicineDouglas Cines - Department of Pathology and Laboratory Medicine, School of MedicineAnn Rux - Department of Microbiology, School of Dental MedicineSharon Willis - Department of Microbiology, School of Dental MedicineGary Cohen - Department of Microbiology, School of Dental MedicineRoselyn Eisenberg - University of PennsylvaniaIrwin Chaiken - Department of Medicine, School of Medicine
- Publication Details
- Methods (San Diego, Calif.), v 19(2), pp 253-269
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Biology; Drexel University
- Web of Science ID
- WOS:000083250600007
- Scopus ID
- 2-s2.0-0032718745
- Other Identifier
- 991019520543704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology