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Exploring the gel phase of cationic glycylalanylglycine in ethanol/water. I. Rheology and microscopy studies
Journal article   Open access   Peer reviewed

Exploring the gel phase of cationic glycylalanylglycine in ethanol/water. I. Rheology and microscopy studies

Lavenia J. Thursch, David DiGuiseppi, Todd R. Lewis, Reinhard Schweitzer-Stenner and Nicolas J. Alvarez
Journal of colloid and interface science, v 564, pp 499-509
22 Mar 2020
DOI: https://doi.org/10.1016/j.jcis.2019.10.029
PMID: 31883655
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1016/j.jcis.2019.10.029View
Accepted (AM)Open Access (Publisher-Specific),  Open

Abstract

GAG Gel rheology Gelation kinetics Peptide fibrils Self-assembly
[Display omitted] The cationic tripeptide glycylalanylglycine (GAG) self-assembles into long, thick crystalline fibrils in an ethanol/water solution. At sufficiently high concentrations, the fibrils form a volume spanning hydrogel network. We report an extensive rheology and microscopy-based study regarding the self-assembly of GAG in ethanol/water solutions to understand the conditions for fibril formation as well as the thermal stability for future developments of this material. By systematically varying GAG concentration and ethanol fraction, we observe a two-dimensional fibril aggregate phase diagram. Microscopy studies shed light on the shape and size of fibrils as well as the macroscopic packing depending on conditions. The kinetics and evolution of the macroscopic fibril microstructure was investigated using rheology. The mechanism of fibril formation is put into the context of a solubility framework, where ethanol reduces peptide solubility and induces self-assembly. The rate of fibril formation and strength of the gel can be controlled by peptide concentration and ethanol fraction. The faster rate of fibril formation leads to inhomogeneous packing of fibrils denoted by discrete dense fibril clusters. The solubility of the fibrils can be manipulated by temperature making the gel thermo-switchable, a property of interest for biomedical systems.

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Web of Science research areas
Chemistry, Physical
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