Journal article
Exploring the importance of the relative solubility of matrix and analyte in MALDI sample preparation using HPLC
Analytical chemistry (Washington), v 76(17), pp 5157-5164
01 Sep 2004
PMID: 15373456
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
New insight into the role of solubility in the sample preparation process for MALDI MS is reported. Reversed-phase gradient HPLC conditions were developed that enable the analysis of a broad range of analyte polarities with a single method. This HPLC method was used to establish a relative polarity scale for a series of 15 MALDI matrix materials, a set of example peptides, and a series of model polymer materials with a broad range of polarity. Examples of each polymer type within the range of 6000-10,000 were analyzed with six matrixes that cover a broad range of polarity using MALDI TOFMS. With regard to polymer signal-to-noise ratio, the matrix and polymer combinations that had a close match of HPLC retention time produced the best MALDI spectra. Conversely, the matrix and polymer combinations that have a large difference in HPLC retention time produced poor MALDI spectra. The results suggest that there is a relationship between polarity (solubility) and effective MALDI sample preparation. The relative HPLC retention time of an unknown polymer can serve as a starting point for predicting the matrix (or range of matrixes) that would be most effective.
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Details
- Title
- Exploring the importance of the relative solubility of matrix and analyte in MALDI sample preparation using HPLC
- Creators
- Andrew J Hoteling - Research & Development Laboratories, Eastman Kodak Company, Rochester, New York 14650-2132, USAWilliam J ErbRobert J TysonKevin G Owens
- Publication Details
- Analytical chemistry (Washington), v 76(17), pp 5157-5164
- Publisher
- American Chemical Society; Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000223733400052
- Scopus ID
- 2-s2.0-4444378871
- Other Identifier
- 991014878400804721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical