Journal article
Expression Patterns of Placenta Growth Factor in Human Melanocytic Cell Lines
Journal of investigative dermatology, v 115(1), pp 118-123
Jul 2000
PMID: 10886518
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Expression patterns of the angiogenic placenta growth factor and its receptor neuropilin-1 were assessed in normal human melanocytes, SV40T-transformed melanocytes, and melanoma cells derived from primary and metastatic lesions. As determined by reverse transcription–polymerase chain reaction all primary and metastatic melanoma cell lines tested and SV40T-transformed melanocytes coexpressed two placenta growth factor splice variants (placenta growth factor-1 and -2) as well as neuropilin-1 mRNA. Placenta growth factor protein was detected in conditioned media derived from five of eight melanomas and from SV40T-transformed melanocytes. In contrast to melanoma cells, normal melanocytes did not express placenta growth factor mRNA at detectable levels and did not secrete placenta growth factor protein. By contrast, neuropilin-1 transcripts were detected in some of the normal melanocytes. Secretion of placenta growth factor by melanoma cells appeared to be constitutive because it was not affected by the addition of exogenous growth factors including insulin, epidermal growth factor, or basic fibroblast growth factor to culture media. Although melanoma cells expressed both, neuropilin-1 and flt-1, exogenous homodimeric placenta growth factor had no effect on melanoma cell growth. Similarly, placenta growth factor did not induce urokinase-type plasminogen activator production in these cells. These findings demonstrate that melanoma progression is accompanied by deregulated, constitutive placenta growth factor expression. Placenta growth factor, however, serves no apparent autocrine role in melanoma proliferation. Further studies are needed to define the relative contribution of placenta growth factor to the angiogenic properties of human melanomas.
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Details
- Title
- Expression Patterns of Placenta Growth Factor in Human Melanocytic Cell Lines
- Creators
- Ullrich GraevenSonja KarpinskiNiko AndreWolff SchmiegelUlrich Rodeck - Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USAMonika Jost - Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
- Publication Details
- Journal of investigative dermatology, v 115(1), pp 118-123
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Intensive Medical Sciences (IMS)
- Web of Science ID
- WOS:000088302900019
- Scopus ID
- 2-s2.0-0033911339
- Other Identifier
- 991014878189004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Dermatology