Journal article
Expression and functional activity of the IL-8 receptor type CXCR1 and CXCR2 on human mast cells
The Journal of immunology (1950), v 161(5), pp 2600-2608
01 Sep 1998
PMID: 9725262
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To further elucidate mechanisms involved in mast cell accumulation at sites of cutaneous inflammation, we have studied the ability of human leukemic mast cells (HMC-1 cells) to express functionally active IL-8 receptors. Expression of mRNA for both types of IL-8 receptors (CXCR1 and CXCR2) was demonstrated by PCR and of both proteins by flow cytometry. Binding and competition studies with 125I-labeled IL-8 and its homologue melanoma growth stimulating activity (125I-labeled MGSA) revealed two specific binding sites for IL-8, K1 = 1.1 x 10(11) M(-1) and K2 = 5 x 10(7) M(-1); and for MGSA, K1 = 2.8 x 10(10) M(-1) and K2 = 5 x 10(7) M(-1). This finding was supported by a dose-dependent rise of cytosolic free calcium concentration ([Ca2+]i) induced by both chemokines and to a lesser extent by the homologue neutrophil-activating peptide-2 (NAP-2). A significant migratory response of human leukemic mast cells (HMC-1) was observed with all three chemokines at a range from 10(-8) M to 10(-9) M. Moreover, the formation of cellular F-actin was induced in a rapid, dose-dependent fashion, with a maximally 1.7-fold increase at 10(-7) M. Using postembedding immunoelectron microscopy, we could show the expression of CXCRI on the cytoplasmatic membrane of isolated human skin mast cells whereas CXCR2 was located in mast cell-specific granules. These findings demonstrate for the first time the functional expression of both types of IL-8 receptors on human mast cells, suggesting a role for their ligands during mast cell activation and recruitment.
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Details
- Title
- Expression and functional activity of the IL-8 receptor type CXCR1 and CXCR2 on human mast cells
- Creators
- U Lippert - Department of Dermatology, Charité, Campus Virchow Klinikum, Humboldt Universität, Berlin, GermanyM ArtucA GrützkauA MöllerA Kenderessy-SzaboD SchadendorfJ NorgauerK HartmannR Schweitzer-StennerT ZuberbierB M HenzS Krüger-Krasagakes
- Publication Details
- The Journal of immunology (1950), v 161(5), pp 2600-2608
- Publisher
- United States
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000075511600067
- Scopus ID
- 2-s2.0-0032167606
- Other Identifier
- 991014877670704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology