Files and links (1)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Expression of GABA(A) receptor alpha(1) subunit mRNA and protein in rat neocortex following photothrombotic infarction
Brain research, v 1210, pp 29-38
19 May 2008
PMID: 18407248
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Photothrombotic infarcts of the neocortex result in structural and functional alterations of cortical networks, including decreased GABAergic inhibition, and can generate epileptic seizures within 1 month of lesioning. In our study, we assessed the involvement and potential changes of cortical GABA(A) receptor (GABA(A)R) alpha(1) subunits at 1, 3, 7, and 30 days after photothrombosis. Quantitative competitive reverse transcription-polymerase chain reaction (cRT-PCR) and semi-quantitative Western blot analysis were used to investigate GABA(A)R alpha(1) subunit mRNA and protein levels in proximal and distal regions of perilesional cortex and in homotopic areas of young adult Sprague-Dawley rats. GABA(A)R alpha(1) subunit mRNA levels were decreased ipsilateral and contralateral to the infarct at 7 days, but were increased bilaterally at 30 days. GABA(A)R alpha(1) subunit protein levels revealed no significant change in neocortical areas of both hemispheres of lesioned animals compared with protein levels of sham-operated controls at 1, 3, 7, and 30 days. At 30 days, GABA(A)R alpha(1) subunit protein expression was significantly increased in lesioned animals within proximal and distal regions of perilesional cortex compared with distal neocortical areas contralaterally (Student's t-test, p<0.05). Short- and long-term alterations of mRNA and protein levels of the GABA(A)R alpha(1) subunit ipsilateral and contralateral to the lesion may influence alterations in cell surface receptor subtype expression and GABA(A)R function following ischemic infarction and maybe associated with formative mechanisms of poststroke epileptogenesis. (C) 2008 Elsevier B.V. All rights reserved.
Metrics
Details
- Title
- Expression of GABA(A) receptor alpha(1) subunit mRNA and protein in rat neocortex following photothrombotic infarction
- Creators
- Elena A. Kharlamov - Allegheny General HospitalKathy L. Downey - Allegheny General HospitalPeter I. Jukkola - Allegheny General HospitalDennis R. Grayson - University of Illinois at ChicagoKevin M. Kelly - Allegheny General Hospital
- Publication Details
- Brain research, v 1210, pp 29-38
- Publisher
- Elsevier
- Number of pages
- 10
- Grant note
- R01 NS046015-03; R01 NS046015; R01NS04601; R01 NS050582-04 / NINDS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R01NS046015 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurology
- Web of Science ID
- WOS:000256507200004
- Scopus ID
- 2-s2.0-42749093047
- Other Identifier
- 991019168829604721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences