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Journal article
Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942
Frontiers in plant science, v 11, pp 231-231
03 Mar 2020
PMID: 32194605
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
An excess of reactive oxygen species (ROS) can cause severe oxidative damage to cellular components in photosynthetic cells. Antioxidant systems, such as the glutathione (GSH) pools, regulate redox status in cells to guard against such damage. Dehydroascorbate reductase (DHAR, EC 1.8.5.1) catalyzes the glutathione-dependent reduction of oxidized ascorbate (dehydroascorbate) and contains a redox active site and glutathione binding-site. The
DHAR
gene is important in biological and abiotic stress responses involving reduction of the oxidative damage caused by ROS. In this study, transgenic
Synechococcus elongatus
PCC 7942 (TA) was constructed by cloning the
Oryza sativa
L.
japonica DHAR
(
OsDHAR
) gene controlled by an isopropyl β-
D
-1-thiogalactopyranoside (IPTG)-inducible promoter (
Ptrc
) into the cyanobacterium to study the functional activities of
OsDHAR
under oxidative stress caused by hydrogen peroxide exposure.
OsDHAR
expression increased the growth of
S. elongatus
PCC 7942 under oxidative stress by reducing the levels of hydroperoxides and malondialdehyde (MDA) and mitigating the loss of chlorophyll. DHAR and glutathione
S
-transferase activity were higher than in the wild-type
S. elongatus
PCC 7942 (WT). Additionally, overexpression of
OsDHAR
in
S. elongatus
PCC 7942 greatly increased the glutathione (GSH)/glutathione disulfide (GSSG) ratio in the presence or absence of hydrogen peroxide. These results strongly suggest that
DHAR
attenuates deleterious oxidative effects via the glutathione (GSH)-dependent antioxidant system in cyanobacterial cells. The expression of heterologous
OsDHAR
in
S. elongatus
PCC 7942 protected cells from oxidative damage through a GSH-dependent antioxidant system via GSH-dependent reactions at the redox active site and GSH binding site residues during oxidative stress.
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Details
- Title
- Expression of Heterologous OsDHAR Gene Improves Glutathione (GSH)-Dependent Antioxidant System and Maintenance of Cellular Redox Status in Synechococcus elongatus PCC 7942
- Creators
- Young-Saeng Kim - Kyungpook National UniversitySeong-Im Park - Kyungpook National UniversityJin-Ju Kim - Kyungpook National UniversityJoseph S. Boyd - University of California, San DiegoJoris Beld - Drexel UniversityArnaud Taton - University of California, San DiegoKyoung-In Lee - , , , , , , , , , , , , , ,Il-Sup Kim - Kyungpook National UniversityJames W. Golden - University of California, San DiegoHo-Sung Yoon - Kyungpook National University
- Publication Details
- Frontiers in plant science, v 11, pp 231-231
- Publisher
- Frontiers Media S.A
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000524769900001
- Scopus ID
- 2-s2.0-85082695113
- Other Identifier
- 991019168825304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Plant Sciences