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Journal article
Expression of an antigen homologous to the human CO17-1A/GA733 colon cancer antigen in animal tissues
British journal of cancer, v 76(7), pp 909-916
01 Jan 1997
PMID: 9328151
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The CO17-1A/GA733 antigen is associated with human carcinomas and some normal epithelial tissues. This antigen has shown promise as a target in approaches to passive and active immunotherapy of colorectal cancer. The relevance of animal models for studies of immunotherapy targeting this antigen in patients is dependent on the expression of the antigen on normal animal tissues. Immunohistoperoxidase staining with polyclonal rabbit antibodies to the human antigen revealed the human homologue on normal small intestine, colon and liver of mice, rats and non-human primates, whereas mouse monoclonal antibodies to the CO17-1A or GA733 epitopes on the human antigen did not detect the antigen. Polyclonal rabbit antibodies, elicited by the murine antigen homologue derived from recombinant baculovirus-infected insect cells, immunoprecipitated the antigen from mouse small intestine, colon, stomach, kidney and lung. The isolated recombinant murine protein bound polyclonal, but not monoclonal, antibodies to the human CO17-1A/GA733 antigen, and recombinant human antigen bound polyclonal antibodies elicited by the murine antigen homologue. Thus, the antigen homologue expressed by animal tissues is similar, but not identical, to the human antigen. These results have important implications for experimental active and passive immunotherapy targeting the CO17-1A/GA733 antigen.
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Details
- Title
- Expression of an antigen homologous to the human CO17-1A/GA733 colon cancer antigen in animal tissues
- Creators
- J. Zaloudik - The Wistar InstituteS. Basak - The Wistar InstituteM. Nesbit - The Wistar InstituteD. W. Speicher - The Wistar InstituteW. H. Wunner - The Wistar InstituteE. Miller - The Wistar InstituteC. Ernst-Grotkowski - Medical College of PhiladelphiaR. Kennedy - University of OklahomaL. P. Bergsagel - Hematology/OncologyT. Koido - The Wistar InstituteD. Herlyn - The Wistar Institute
- Publication Details
- British journal of cancer, v 76(7), pp 909-916
- Publisher
- Nature Publishing Group
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Psychological and Brain Sciences (Psychology)
- Web of Science ID
- WOS:A1997XZ11900013
- Scopus ID
- 2-s2.0-9844242130
- Other Identifier
- 991019168971004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Oncology