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Expression of endothelial cell adhesion molecules in neovascularized tissue
Journal article   Peer reviewed

Expression of endothelial cell adhesion molecules in neovascularized tissue

G Vallien, R Langley, S Jennings, R Specian and D N Granger
Microcirculation (New York, N.Y. 1994), v 7(4), pp 249-258
Aug 2000
DOI: https://doi.org/10.1038/sj.mn.7300113
PMID: 10963630
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Endothelium, Vascular - cytology Immunohistochemistry Antigens, CD - immunology Neovascularization, Physiologic - drug effects Vascular Cell Adhesion Molecule-1 - drug effects Antigens, CD - biosynthesis Endothelium, Vascular - drug effects Male Cell Adhesion Molecules - immunology Endothelium, Vascular - chemistry Platelet Endothelial Cell Adhesion Molecule-1 - immunology Intercellular Adhesion Molecule-1 - biosynthesis E-Selectin - biosynthesis Intercellular Adhesion Molecule-1 - drug effects E-Selectin - drug effects Cell Adhesion Molecules - drug effects P-Selectin - immunology Vascular Cell Adhesion Molecule-1 - immunology Cell Adhesion Molecules - biosynthesis Intercellular Adhesion Molecule-1 - immunology Mice, Inbred C57BL E-Selectin - immunology Antigens, CD - drug effects Tumor Necrosis Factor-alpha - pharmacology Phenotype Animals P-Selectin - drug effects P-Selectin - biosynthesis Platelet Endothelial Cell Adhesion Molecule-1 - drug effects Vascular Cell Adhesion Molecule-1 - biosynthesis Mice Platelet Endothelial Cell Adhesion Molecule-1 - biosynthesis
Recent studies indicate that endothelial cells of newly formed blood vessels are activated and exhibit a distinct phenotype that may influence the responses of these microvessels to an inflammatory stimulus. The objective of this study was to compare the basal and cytokine-stimulated expression of endothelial cell adhesion molecules in neovascularized tissue to normal (nonproliferating) vascular beds. The expression of P- and E-selectin. VCAM-1, ICAM-1, ICAM-2, and PECAM-1 was measured, using the dual radiolabeled mAb technique, in subcutaneously implanted (for 10-15 days) polyurethane sponges, skin, heart, lung, and intestine of male C57BL/6 mlice (background). Basal values of PECAM-1 and ICAM-2 revealed a low vascular density in the implanted sponge matrices that is comparable to skin. When normalized for vascular surface area (PECAM-1 or ICAM-1 expression), the basal level of E- and P-selectin expression was highest in neovascularized sponge and skin. TNF-alpha elicited an increased expression of all endothelial CAMs, except PECAM-1 and ICAM-2, but the responses were blunted in sponge and skin, relative to other vascular beds. These findings indicate that endothelial cells in newly formed blood vessels exhibit a pattern of basal and cytokine-induced expression of certain adhesion glycoproteins that is similar to nonproliferating cutaneous vessels.

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Web of Science research areas
Hematology
Peripheral Vascular Disease
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