Journal article
Expression of interfering RNAs from an HIV-1 Tat-inducible chimeric promoter
Virus research, v 155(1), pp 106-111
Jan 2011
PMID: 20851721
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The therapeutic value of antiviral interfering RNAs could be improved by technologies that limit their expression to the infected cell population. The HIV-1 Tat-inducible viral LTR and LTR-containing chimeric promoters have previously been used to drive expression of antiviral RNAs and proteins directed against HIV-1. Here, we characterize an alternative promoter, consisting of a chicken β-actin core promoter fused to the viral TAR element, for the conditional expression of interfering RNAs. This promoter, that we refer to as the CK-TAR promoter, can induce levels of silencing comparable to the viral LTR in response to Tat produced from co-transfected plasmids or during viral replication. While the CK-TAR promoter shows a modest level of basal activity, similar to the viral LTR, it is less responsive to the extracellular stimuli tested including LPS, TNFα, and PMA. The CK-TAR promoter is an alternative Tat-inducible promoter with the potential to minimize the risk of vector mobilization and to drive polycistronic expression of interfering RNAs.
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Details
- Title
- Expression of interfering RNAs from an HIV-1 Tat-inducible chimeric promoter
- Creators
- Viraj R Sanghvi - Department of Microbiology and Immunology, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, 245 North 15th Street, Philadelphia, PA 19102, USALaura F Steel
- Publication Details
- Virus research, v 155(1), pp 106-111
- Publisher
- Elsevier; Netherlands
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000287010300015
- Scopus ID
- 2-s2.0-78650550809
- Other Identifier
- 991014877827004721
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- Web of Science research areas
- Virology