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Expression of latent HAART-persistent HIV type 1 induced by novel cellular activating agents
Journal article   Peer reviewed

Expression of latent HAART-persistent HIV type 1 induced by novel cellular activating agents

Joseph Kulkosky, Julie Sullivan, Yan Xu, Emily Souder, Dean H Hamer and Roger J Pomerantz
AIDS research and human retroviruses, v 20(5), pp 497-505
01 May 2004
PMID: 15186524

Abstract

Amino Acid Sequence Antiretroviral Therapy, Highly Active CD4-Positive T-Lymphocytes - cytology HIV Core Protein p24 - pharmacology HIV Infections - immunology HIV Infections - virology HIV-1 - drug effects Humans Molecular Sequence Data Muromonab-CD3 - pharmacology Phorbol Esters - pharmacology Phytohemagglutinins - pharmacology
The novel antitumor-promoting phorbol ester, prostratin, was evaluated for its ability to induce the expression of latent, highly active antiretroviral therapy (HAART)-persistent human immunodeficiency virus type I (HIV-1) from specific subsets of patients' peripheral blood cells. This evaluation was performed relative to the use of other cellular activating agents, such as OKT3, a monoclonal antibody against the human T cell receptor, interleukin-2 (IL-2), phytohemagglutinin (PHA), p24 antigen (HIV-1-specific capsid protein), and a molecular relative of prostratin, 12-deoxyphorbol 13-phenylacetate (DPP). Prostratin performed as efficiently as the other cellular activators at inducing the expression of latent HIV-1 from cells of patients on virally suppressive HAART. Of interest was the induction of a novel species of latent virus from the cells of an individual after exposure to the HIV-1-specific capsid protein, p24, relative to virus expression induced by several other cell activators. This suggests that a variety of agents may be available for animal model studies of lentiviral latency and clinical use to broadly induce the expression of latent, HAART-persistent HIV-1 in vivo with the goal of potential HIV-1 reservoir depletion or eradication.

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Collaboration types
Domestic collaboration
Web of Science research areas
Immunology
Infectious Diseases
Virology
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