Journal article
Extracellular Tax-protein of human T cell leukemia virus type 1 induces the secretion of Th1 cytokines and beta-chemokines from monocyte-derived dendritic cells
Journal of neurovirology, Vol.12, pp.44-44
01 May 2006
Abstract
HTLV-1-associated myelopathy/tropical spastic paraparesis, a neurological disorder, is characterized by highly stimulated immune response including elevated levels of inflammatory cytokines/chemokines, and oligoclonal expansion of Tax-specific CD8+ cytotoxic T lymphocytes in the peripheral blood and cerebrospinal fluid. Studies have shown that HTLV-1 transactivator protein Tax is available for immune recognition by antigen presenting cells such as dendritic cells, the most efficient APCs. In this study we have shown that the treatment of monocyte-derived dendritic cells (MDDCs) with purified Tax protein induces the secretion of Th1 cytokines (IL-12, and TNF-alpha) and beta-chemokines (MIP-Ialpha, MIP-Ibeta, and RANTES) in a dose- and time-dependent manner as quantitated by antigen-specific ELISA. The kinetics and dose response studies were also performed at mRNA level by real-time PCR. A significant correlation has been observed in the Tax-induced secretion of cytokines/chemokines at both RNA and protein levels. These studies suggest that HTLV-1 Tax protein can initiate immune response by activating DCs, the mechanism(s) of which is currently under investigation.
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Details
- Title
- Extracellular Tax-protein of human T cell leukemia virus type 1 induces the secretion of Th1 cytokines and beta-chemokines from monocyte-derived dendritic cells
- Creators
- V LepoutreP JainJ AhujaB Wigdahl
- Publication Details
- Journal of neurovirology, Vol.12, pp.44-44
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170461304721