Journal article
Extraction and Analysis of Extracellular Polymeric Substances: Comparison of Methods and Extracellular Polymeric Substance Levels in Salmonella pullorum SA 1685
Environmental engineering science, v 26(10), pp 1523-1532
01 Oct 2009
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Extracellular polymeric substances (EPS) production and composition for Salmonella pullorum SA 1685 exposed to artificial groundwater (AGW) has been examined utilizing three EPS extraction methods: lyophilization, ethanol, and sonication. Experiments were carried out to evaluate the robustness of three EPS extraction methods and the sensitivity of each to subtle changes in solution ionic strength (IS) and duration of exposure. EPS extraction and analysis was conducted via sugar and protein analyses using the phenol sulfuric acid and Lowry methods, respectively, after 0-, 6-, 12-, 18-, and 24-h incubation times in AGW with 10(-2.5), 10(-2), and 10(-1.5) M IS. Lyophilization and ethanol methods resulted in a greater amount of EPS extracted than the sonication method (mass of EPS/cell), yet these methods fluctuated to a greater extent in the total amount-or level-of EPS extracted under the various test conditions. Systematic comparisons and extensive statistical analyses were conducted between the various experimental conditions. To our knowledge, this is the first study systematically comparing EPS extraction techniques utilizing Salmonella. As we investigated the relative EPS content in Salmonella SA1685 under conditions simulating groundwater, our results provide insight into the suitability of each method for detection of environmentally induced changes in bacteria suspended in simulated or real subsurface aquatic systems.
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Details
- Title
- Extraction and Analysis of Extracellular Polymeric Substances: Comparison of Methods and Extracellular Polymeric Substance Levels in Salmonella pullorum SA 1685
- Creators
- Amy S. Gong - University of California, RiversideCarl H. Bolster - Agricultural Research ServiceMagda Benavides - University of California, RiversideSharon L. Walker - University of California, Riverside
- Publication Details
- Environmental engineering science, v 26(10), pp 1523-1532
- Publisher
- Mary Ann Liebert, Inc
- Number of pages
- 10
- Grant note
- American Society for Microbiology, Microbiology Undergraduate Research Fellowship P200A060047 / U. S. Department of Education GAANN; US Department of Education EEC0649096 / National Science Foundation REU program; National Science Foundation (NSF) 05-WQ-005 / National Water Research Institute
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000270822200008
- Scopus ID
- 2-s2.0-70449477004
- Other Identifier
- 991021229900204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Environmental
- Environmental Sciences