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Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
FANCD2 limits acetaldehyde-induced genomic instability during DNA replication in esophageal keratinocytes
Molecular oncology, v 15(11), pp 3109-3124
Nov 2021
PMID: 34328261
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Individuals with Fanconi anemia (FA), a rare genetic bone marrow failure syndrome, have an increased risk of young-onset head and neck squamous cell carcinomas (SCCs) and esophageal SCC. The FA DNA repair pathway is activated upon DNA damage induced by acetaldehyde, a chief alcohol metabolite and one of the major carcinogens in humans. However, the molecular basis of acetaldehyde-induced genomic instability in SCCs of the head and neck and of the esophagus in FA remains elusive. Here, we report the effects of acetaldehyde on replication stress response in esophageal epithelial cells (keratinocytes). Acetaldehyde-exposed esophageal keratinocytes displayed accumulation of DNA damage foci consisting of 53BP1 and BRCA1. At physiologically relevant concentrations, acetaldehyde activated the ATR-Chk1 pathway, leading to S- and G2/M-phase delay with accumulation of the FA complementation group D2 protein (FANCD2) at the sites of DNA synthesis, suggesting that acetaldehyde impedes replication fork progression. Consistently, depletion of the replication fork protection protein Timeless led to elevated DNA damage upon acetaldehyde exposure. Furthermore, FANCD2 depletion exacerbated replication abnormalities, elevated DNA damage, and led to apoptotic cell death, indicating that FANCD2 prevents acetaldehyde-induced genomic instability in esophageal keratinocytes. These observations contribute to our understanding of the mechanisms that drive genomic instability in FA patients and alcohol-related carcinogenesis, thereby providing a translational implication in the development of more effective therapies for SCCs.
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Details
- Title
- FANCD2 limits acetaldehyde-induced genomic instability during DNA replication in esophageal keratinocytes
- Creators
- Jasmine D Peake - Drexel UniversityChiaki Noguchi - Drexel UniversityBaicheng Lin - Drexel UniversityAmber Theriault - Drexel UniversityMargaret O'Connor - Drexel UniversityShivani Sheth - Drexel UniversityKoji Tanaka - University of PennsylvaniaHiroshi Nakagawa - Columbia UniversityEishi Noguchi - Drexel University
- Publication Details
- Molecular oncology, v 15(11), pp 3109-3124
- Publisher
- Wiley
- Grant note
- P01 CA098101 / NCI NIH HHS 27307C2007 / NIEHS NIH HHS P30 CA013696 / NCI NIH HHS R01 AA026297 / NIAAA NIH HHS F31 AA027133 / NIAAA NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Hematology and Oncology
- Web of Science ID
- WOS:000682735600001
- Scopus ID
- 2-s2.0-85112617483
- Other Identifier
- 991019168493904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Oncology