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FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques
Journal article   Open access   Peer reviewed

FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques

Rajnish S Dave, Ravi K Sharma, Roshell R Muir, Elias Haddad, Sanjeev Gumber, Francois Villinger, Artinder P Nehra, Zafar K Khan, Brian Wigdahl, Aftab A Ansari, …
Journal of neuroimmune pharmacology, v 13(2), pp 204-218
Jun 2018
PMID: 29288344
url
https://europepmc.org/articles/pmc5757373View

Abstract

Simian Acquired Immunodeficiency Syndrome - virology Animals Dendritic Cells, Follicular - immunology Simian Acquired Immunodeficiency Syndrome - immunology T-Lymphocytes, Helper-Inducer - immunology Dendritic Cells, Follicular - virology Lymph Nodes - virology Macaca mulatta Lymph Nodes - immunology T-Lymphocytes, Helper-Inducer - virology
Cerebrospinal fluid (CSF) drains via the lymphatic drainage pathway. This lymphatic pathway connects the central nervous system (CNS) to the cervical lymph node (CLN). As the CSF drains to CLN via the dural and nasal lymphatics, T cells and antigen presenting cells pass along the channels from the subarachnoid space through the cribriform plate. Human immunodeficiency virus (HIV) may also egress from the CNS along this pathway. As a result, HIV egressing from the CNS may accumulate within the CLN. Towards this objective, we analyzed CLNs isolated from rhesus macaques that were chronically-infected with simian immunodeficiency virus (SIV). We detected significant accumulation of SIV within the CLNs. SIV virion trapping was observed on follicular dendritic cells (FDCs) localized within the follicular regions of CLNs. In addition, SIV antigens formed immune complexes when FDCs interacted with B cells within the germinal centers. Subsequent interaction of these B cells with CD4+ T follicular helper cells (T) resulted in infection of the latter. Of note, 73% to 90% of the T cells within CLNs were positive for SIV p27 antigen. As such, it appears that not only do the FDCs retain SIV they also transmit them (via B cells) to T within these CLNs. This interaction results in infection of T in the CLNs. Based on these observations, we infer that FDCs within the CLNs have a novel role in SIV entrapment with implications for viral trafficking.

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Collaboration types
Domestic collaboration
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Web of Science research areas
Neurosciences
Pharmacology & Pharmacy
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