Journal article
Factors Determining the Success of the Chronically Instrumented Non-anesthetized Fetal Sheep Model of Human Development: A Retrospective Cohort Study
CUREUS JOURNAL OF MEDICAL SCIENCE, v 14(12), e32632
17 Dec 2022
PMID: 36660509
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background The chronically instrumented non-anesthetized fetal sheep (CINAFS) model has been a mainstay of human fetal development research for the past 60 years. As a large two for one animal model, involving the instrumentation of the ewe and her fetus, the model poses challenges to implement de novo and maintain overtime at the highest standards of operating procedures to ensure ongoing performance. A common yet conventionally underreported issue researchers face is a high rate of animal loss. Here, we investigate what determines the success of the CINAFS model of human development. Methods We conducted a retrospective cohort analysis consisting of 82 experiments spanning the course of six years. Our team identified 10 variables that we anticipated were likely to influence the experimental outcome, such as the time of year, animal size, and surgical complexity. To evaluate the role of each variable in contributing to the success of the model, a binary logit regression analysis with a Fisher scoring optimization was fit to the data (SAS, V9 engine, release 3.8, SAS Institute, Cary, NC, USA). A higher predictive probability indicates a larger impact by the given variable on the outcome of the experiment. A Wald chi-squared analysis was run on the data to control for confounders and determine significance. Results The single variable identified in this study as determining the success of experiment outcomes using the CINAFS model is the experience level of the team. Conclusion . The CINAFS model offers enormous potential to further our understanding of human fetal development and create interventional technologies related to fetal health. However, to improve experimental outcomes using the CINAFS model, stronger communication and training are needed. We discuss the implications of our findings for the successful implementation of this challenging yet scientifically advantageous animal model of human physiology.
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- Title
- Factors Determining the Success of the Chronically Instrumented Non-anesthetized Fetal Sheep Model of Human Development: A Retrospective Cohort Study
- Publication Details
- CUREUS JOURNAL OF MEDICAL SCIENCE, v 14(12), e32632
- Publisher
- CUREUS INC; PALO ALTO
- Grant note
- Acknowledgements Funding: This study is funded by the Canadian Institutes of Health Research (CIHR) , Fonds de recherche du Quebec-Sante (FRQS) , Molly Towell Perinatal Research Foundation (to MGF) . Data availability declaration: All available data is included in the manuscript figures. Acknowledgments: The authors gratefully acknowledge technical support by Esther Simard, Marco Bosa, Pierre-Yves Mulon, and L. Daniel Durosier during the experiments. The authors thank the amazing Clinical Sciences team of the Facult? m?decine v?t?rinaire at the Universit? de Montr?al for the skillful assistance throughout the experiments. The authors also gratefully acknowledge the statistical support by Miguel Chagnon.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Drexel University
- Web of Science ID
- WOS:000905174000003
- Other Identifier
- 991021861201704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Neurosciences