Journal article
Fatty Acid Amide Biosynthesis: A Possible New Role for Peptidylglycine α-Amidating Enzyme and Acyl-Coenzyme A:GlycineN-Acyltransferase
Archives of biochemistry and biophysics, v 330(2), pp 430-434
15 Jun 1996
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Abstract
Fatty acid primary amides have recently been recognized as mammalian hormones [Cravattet al.(1995)Science268, 1506–1509]. The route to their biosynthesis is unknown. Many mammalian peptide hormones also possess a C-terminal α-amide moiety that arises from the posttranslational oxidative cleavage of a C-terminal glycine-extended precursor. The enzyme that catalyzes this reaction is peptidylglycine α-amidating enzyme, which is known to preferentially amidate peptide substrates containing a penultimate, hydrophobic amino acid [Tamburiniet al.(1990)Int. J. Pept. Protein Res.35, 153–156]. We show thatN-myristoylglycine is a substrate for peptidylglycine α-amidating enzyme with a (V/K)appthat is 55 ± 4% of the value measured for D-Tyr-Val-Gly.N-Fatty acylglycines are enzymatically produced in mammals from fatty acyl-coenzyme A (CoAs) and glycine by acyl-CoA:glycineN-acyltransferase. The sequential actions of acyl-CoA:glycineN-acyltransferase and peptidylglycine α-amidating enzyme would lead to the biosynthesis of fatty acid amides.
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Details
- Title
- Fatty Acid Amide Biosynthesis: A Possible New Role for Peptidylglycine α-Amidating Enzyme and Acyl-Coenzyme A:GlycineN-Acyltransferase
- Creators
- David J. Merkler - Duquesne UniversityKathleen A. Merkler - Duquesne UniversityWilliam Stern - Protein Chemistry Group, Unigene Laboratories, Inc. 110 Little Falls Road, Fairfield, New Jersey, 07004Fraser F. Fleming - Duquesne University
- Publication Details
- Archives of biochemistry and biophysics, v 330(2), pp 430-434
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:A1996UV56600026
- Scopus ID
- 2-s2.0-0030008554
- Other Identifier
- 991020898392704721
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics