Journal article
Ferrocyanide-Mediated Photoreduction of Ferricytochrome C Utilized to Selectively Probe Non-native Conformations Induced by Binding to Cardiolipin-Containing Liposomes
Chemistry : a European journal, v 23(5), pp 1151-1156
01 Jan 2017
PMID: 27859757
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Ferricytochrome c binding to cardiolipin-containing liposomes produces a heterogeneous distribution of conformations comprising native-like and non-native misfolded proteins. We utilized the photoreduction of native ferricytochrome c in the presence of potassium ferrocyanide and resonance Raman spectroscopy to probe the population of native and misfolded cytochrome c on liposomes with 20% tetraoleylcardiolipin (TOCL)/80% dioleylphosphocholine (DOPC) and with 100% TOCL as a function of TOCL concentration. Our data provided strong support for an earlier model, which predicts that the equilibrium between native and non-native conformations is shifted to the latter with decreasing protein occupation of liposomes.
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Details
- Title
- Ferrocyanide-Mediated Photoreduction of Ferricytochrome C Utilized to Selectively Probe Non-native Conformations Induced by Binding to Cardiolipin-Containing Liposomes
- Creators
- Dmitry Malyshka - Drexel UniversityReinhard Schweitzer-Stenner - Drexel University
- Publication Details
- Chemistry : a European journal, v 23(5), pp 1151-1156
- Publisher
- Wiley
- Number of pages
- 6
- Grant note
- Department of Chemistry
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000395753000027
- Scopus ID
- 2-s2.0-85007228378
- Other Identifier
- 991019167787004721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Multidisciplinary