Files and links (1)
Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma
Nature communications, v 11(1), pp 1435-1435
18 Mar 2020
PMID: 32188843
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Regeneration of corneal stroma has always been a challenge due to its sophisticated structure and keratocyte-fibroblast transformation. In this study, we fabricate grid poly (ε-caprolactone)-poly (ethylene glycol) microfibrous scaffold and infuse the scaffold with gelatin methacrylate (GelMA) hydrogel to obtain a 3 D fiber hydrogel construct; the fiber spacing is adjusted to fabricate optimal construct that simulates the stromal structure with properties most similar to the native cornea. The topological structure (3 D fiber hydrogel, 3 D GelMA hydrogel, and 2 D culture dish) and chemical factors (serum, ascorbic acid, insulin, and β-FGF) are examined to study their effects on the differentiation of limbal stromal stem cells to keratocytes or fibroblasts and the phenotype maintenance, in vitro and in vivo tissue regeneration. The results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environment for the maintenance of keratocyte phenotype and the regeneration of damaged corneal stroma.
Metrics
Details
- Title
- Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma
- Creators
- Bin Kong - Tsinghua–Berkeley Shenzhen InstituteYun Chen - Open FIESTA Center, Tsinghua Shenzhen International Graduate School, 518055, Shenzhen, P.R. China.Rui Liu - Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School, 518055, Shenzhen, P.R. China.Xi Liu - Beijing Children’s HospitalChangyong Liu - Manufacturing InstituteZengwu Shao - Tongji Medical CollegeLiming Xiong - Tongji Medical CollegeXianning Liu - Shaanxi Key Laboratory of Eye, 710002, Xi'an, P.R. ChinaWei Sun - Drexel UniversityShengli Mi - Open FIESTA Center, Tsinghua Shenzhen International Graduate School, Shenzhen, P.R. China
- Publication Details
- Nature communications, v 11(1), pp 1435-1435
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000522032300012
- Scopus ID
- 2-s2.0-85081996747
- Other Identifier
- 991019167808704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
Highly Cited Paper
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical