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Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge
Journal article   Open access   Peer reviewed

Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge

Zunlu Hu, Jie Feng, Weijuan Bo, Ronghua Wu, Zhangji Dong, Yan Liu, Liang Qiang and Mei Liu
Molecular biology of the cell, v 28(4), pp 545-553
15 Feb 2017
DOI: https://doi.org/10.1091/mbc.E16-09-0628
PMID: 27974640
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1091/mbc.e16-09-0628View
Published, Version of Record (VoR)CC BY-NC-SA V4.0,  Open

Abstract

Adenosine Triphosphatases - metabolism Adenosine Triphosphatases - physiology Animals Astrocytes - metabolism ATPases Associated with Diverse Cellular Activities Cell Movement - physiology Cells, Cultured Kinesin - metabolism Microtubule-Associated Proteins Microtubules - metabolism Neurogenesis Neurons - metabolism Nuclear Proteins - metabolism Nuclear Proteins - physiology Rats Tubulin - metabolism Tyrosine
Microtubule (MT) organization is essential for many cellular events, including mitosis, migration, and cell polarity. Fidgetin (Fign), an ATP-dependent, MT-severing protein, contributes to the regulation of MT configuration by cutting and trimming MT polymers. Functions of Fign have been indicated in neurite outgrowth, mitosis, meiosis, and cellular migration. Here we focus on migration of astrocytes. We find that Fign plays an essential role in cultured astrocyte migration by preferentially targeting MTs (or regions of MTs) that are rich in tyrosinated tubulin, a marker for especially dynamic MTs or especially dynamic regions of MTs. Inhibition of cellular migration induced by Fign knockdown can be rescued with concomitant knockdown of kinesin-12, a motor protein best known for its role in mitosis. We propose a novel working model for MT reconfiguration underlying cellular migration elicited by the functional cooperation of two distinct MT-related proteins.

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36 citations in Web of Science
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Web of Science research areas
Cell Biology
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