Journal article
Fine-mapping quantitative trait loci affecting murine external ear tissue regeneration in the LG/J by SM/J advanced intercross line
Heredity, v 112(5), pp 508-518
May 2014
PMID: 24569637
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
External ear hole closure in LG/J mice represents a model of regenerative response. It is accompanied by the formation of a blastema-like structure and the re-growth of multiple tissues, including cartilage. The ability to regenerate tissue is heritable. An F34 advanced intercross line of mice (Wustl:LG,SM-G34) was generated to identify genomic loci involved in ear hole closure over a 30-day healing period. We mapped 19 quantitative trait loci (QTL) for ear hole closure. Individual gene effects are relatively small (0.08 mm), and most loci have co-dominant effects with phenotypically intermediate heterozygotes. QTL support regions were limited to a median size of 2 Mb containing a median of 19 genes. Positional candidate genes were evaluated using differential transcript expression between LG/J and SM/J healing tissue, function analysis and bioinformatic analysis of single-nucleotide polymorphisms in and around positional candidate genes of interest. Analysis of the set of 34 positional candidate genes and those displaying expression differences revealed over-representation of genes involved in cell cycle regulation/DNA damage, cell migration and adhesion, developmentally related genes and metabolism. This indicates that the healing phenotype in LG/J mice involves multiple physiological mechanisms.
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Details
- Title
- Fine-mapping quantitative trait loci affecting murine external ear tissue regeneration in the LG/J by SM/J advanced intercross line
- Creators
- J M Cheverud - Washington University in St. LouisH A Lawson - Washington University in St. LouisK Bouckaert - Washington University in St. LouisA V Kossenkov - The Wistar InstituteL C Showe - The Wistar InstituteL Cort - Drexel UniversityE P Blankenhorn - Drexel UniversityK Bedelbaeva - The Wistar InstituteD Gourevitch - The Wistar InstituteY Zhang - The Wistar InstituteE Heber-Katz - The Wistar Institute
- Publication Details
- Heredity, v 112(5), pp 508-518
- Publisher
- Springer Nature
- Grant note
- HHSN268200782096C / NHLBI NIH HHS P30 CA010815 / NCI NIH HHS GM073226 / NIGMS NIH HHS R01 GM073226 / NIGMS NIH HHS HHSN268200782096C / NHGRI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000334605700006
- Scopus ID
- 2-s2.0-84899464011
- Other Identifier
- 991019168543004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Ecology
- Evolutionary Biology
- Genetics & Heredity