Journal article
Finger-Actuated, Self-Contained Immunoassay Cassettes
Biomedical microdevices, v 11(6), pp 1175-1186
Dec 2009
PMID: 19597994
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The building blocks for an inexpensive, disposable, luminescence-based microfluidic immunoassay cassette are described, and their integration in a point-of-care diagnostic system is demonstrated. Fluid motion in the cassette is driven by depressing finger-actuated pouches. All reagents needed for the immunoassay can be stored in the cassette in liquid form. Prior to use, the cassette consists of two separate parts. A top storage component contains pouches, sealed storage chambers, a metering chamber, and needle seats. The bottom processing component contains connection needles, a mixing chamber, and a detection chamber with immobilized proteins. Subsequent to sample introduction, the storage and processing components are mated. The needles form hydraulic connections between the two parts and, in some cases, close valves. The pouches are then actuated sequentially to induce flow of various reagents and facilitate process operations. The cassette is compatible with different detection modalities. Both a cassette with immunochromatographic-based detection and a cassette with microbead-based detection were constructed and evaluated. The immunochromatographic cassette was used to detect antibodies to HIV in saliva samples. The bead-based cassette was used to detect the proinflammatory chemokine IL-8. The experimental data demonstrates good repeatability and reasonable sensitivity.
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Details
- Title
- Finger-Actuated, Self-Contained Immunoassay Cassettes
- Creators
- Xianbo Qiu - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USAJason A Thompson - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USAZongyuan Chen - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USAChangchun Liu - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USADafeng Chen - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USASudhir Ramprasad - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USAMichael G Mauk - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USASerge Ongagna - Department of Basic Sciences, New York University College of Dentistry, New York, 10010, USACheryl Barber - Department of Basic Sciences, New York University College of Dentistry, New York, 10010, USAWilliam R Abrams - Department of Basic Sciences, New York University College of Dentistry, New York, 10010, USADaniel Malamud - Department of Basic Sciences, New York University College of Dentistry, New York, 10010, USAPaul L.A.M Corstjens - Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The NetherlandsHaim H Bau - Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USA
- Publication Details
- Biomedical microdevices, v 11(6), pp 1175-1186
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000271721000004
- Scopus ID
- 2-s2.0-70549105083
- Other Identifier
- 991014878188404721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Nanoscience & Nanotechnology