Journal article
Firing and Cellular Properties of V2a Interneurons in the Rodent Spinal Cord
The Journal of neuroscience, v 30(1), pp 24-37
06 Jan 2010
PMID: 20053884
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Previous studies have shown that a group of ventrally located neurons, designated V2a interneurons, play a key role in maintaining locomotor rhythmicity and in ensuring appropriate left-right alternation during locomotion ( Crone et al., 2008, 2009). These V2a interneurons express the transcription factor Chx10. The aim of the present study was to characterize the locomotor-related activity of individual V2a interneurons, their cellular properties, and their detailed anatomical attributes in Chx10-GFP mice. A dorsal horn-removed preparation was developed to allow for visual whole-cell patch recordings from V2a interneurons along the entire lumbar spinal cord while at the same time leaving enough of the spinal cord intact to generate fictive locomotion. During drug-evoked locomotor-like activity, a large proportion of Chx10 cells showed rhythmic firing or membrane potential fluctuations related to either flexor or extensor activity in every lumbar segment. Chx10 cells received predominantly rhythmic excitatory input. Chx10 neurons displayed a wide variety of firing and potential rhythmogenic properties. However, none of these properties was obviously related to the observed rhythmicity during locomotor-like activity. In dual recordings, we found no evidence of Chx10 neuron interconnectivity. Intracellular fills revealed diverse projection patterns with most Chx10 interneurons being local with projections to the central pattern generator and motor neuron regions of the spinal cord and others with long ascending and/or descending branches. These data are compatible with V2a neurons having a role in regulating segmental left-right alternation and ipsilateral motor neuron firing with little effect on rhythm generation.
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Details
- Title
- Firing and Cellular Properties of V2a Interneurons in the Rodent Spinal Cord
- Creators
- Kimberly J. Dougherty - Karolinska InstitutetOle Kiehn - Karolinska Institutet
- Publication Details
- The Journal of neuroscience, v 30(1), pp 24-37
- Publisher
- Soc Neuroscience
- Number of pages
- 14
- Grant note
- R01 NS040795-08 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Swedish Medical Research Council; Swedish Medical Research Council (SMRC) European Union; European Commission Soderbergs Foundation, Hjarnfonden Friends of Karolinska Institutet 0701166 / Wings for Life, National Science Foundation; National Science Foundation (NSF) R01NS040795 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy; College of Medicine; Drexel University
- Web of Science ID
- WOS:000273347300003
- Scopus ID
- 2-s2.0-74849099437
- Other Identifier
- 991020100077104721
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- Web of Science research areas
- Neurosciences