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Fluorescein Binding to Normal Human Serum Proteins Demonstrated by Equilibrium Dialysis
Journal article

Fluorescein Binding to Normal Human Serum Proteins Demonstrated by Equilibrium Dialysis

Weiye Li and John H Rockey
Archives of ophthalmology (1960), v 100(3), pp 484-487
01 Mar 1982
PMID: 7065970

Abstract

• The binding of fluorescein to normal human serum proteins in a physiologic solvent at 37 °C was measured by equilibrium dialysis. Human serum contained 3.28 x 10-3M concentration fluoresceinbinding sites, with an average association constant at 37 °C of 0.54 X 104M-1. The percentage of total fluorescein bound by human serum proteins ranged from 83% to 53% when the total fluorescein concentration ranged from 6.9 X 10-5 to 6.2 X 10-3M.

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Ophthalmology
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