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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Force dependent internalization of magnetic nanoparticles results in highly loaded endothelial cells for use as potential therapy delivery vectors
Pharmaceutical research, v 29(5), pp 1270-1281
May 2012
PMID: 22234617
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To investigate the kinetics, mechanism and extent of MNP loading into endothelial cells and the effect of this loading on cell function.
MNP uptake was examined under field on/off conditions, utilizing varying magnetite concentration MNPs. MNP-loaded cell viability and functional integrity was assessed using metabolic respiration, cell proliferation and migration assays.
MNP uptake in endothelial cells significantly increased under the influence of a magnetic field versus non-magnetic conditions. Larger magnetite density of the MNPs led to a higher MNP internalization by cells under application of a magnetic field without compromising cellular respiration activity. Two-dimensional migration assays at no field showed that higher magnetite loading resulted in greater cell migration rates. In a three-dimensional migration assay under magnetic field, the migration rate of MNP-loaded cells was more than twice that of unloaded cells and was comparable to migration stimulated by a serum gradient.
Our results suggest that endothelial cell uptake of MNPs is a force dependent process. The in vitro assays determined that cell health is not adversely affected by high MNP loadings, allowing these highly magnetically responsive cells to be potentially beneficial therapy (gene, drug or cell) delivery systems.
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Details
- Title
- Force dependent internalization of magnetic nanoparticles results in highly loaded endothelial cells for use as potential therapy delivery vectors
- Creators
- Cristin MacDonald - Drexel UniversityKenneth Barbee - Drexel UniversityBoris Polyak - Drexel University
- Publication Details
- Pharmaceutical research, v 29(5), pp 1270-1281
- Publisher
- Springer Nature
- Grant note
- F31 GM086128 / NIGMS NIH HHS R01 HL107771 / NHLBI NIH HHS R01HL107771 / NHLBI NIH HHS F31 GM086128-01 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Surgery
- Web of Science ID
- WOS:000303463300010
- Scopus ID
- 2-s2.0-84862661081
- Other Identifier
- 991019168714904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Multidisciplinary
- Pharmacology & Pharmacy