Files and links (2)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Fragile X granules are a family of axonal ribonucleoprotein particles with circuit‐dependent protein composition and mRNA cargos
Journal of comparative neurology (1911), v 526(1), pp 96-108
01 Jan 2018
PMID: 28884477
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Local axonal protein synthesis plays a crucial role in the formation and function of neuronal circuits. Understanding the role of this mechanism in specific circuits requires identifying the protein composition and mRNA cargos of the ribonucleoprotein particles (RNPs) that form the substrate for axonal translation. FXGs (Fragile X granules) are axonal RNPs present in a stereotyped subset of mature axons in the intact brain that contain one or more of the Fragile X related (FXR) proteins (FMRP, FXR2P, and FXR1P) along with mRNA and ribosomes. Here we performed a systematic survey of the FXR protein composition and mRNA association of FXGs in the brain. We have identified four FXG types that can be categorized based on their FXR protein complement. All FXGs contain FXR2P, with FMRP and/or FXR1P present in circuit‐selective subsets. Individual neuronal cell types predominantly express a single FXG type, with FMRP‐containing FXGs the most prevalent in forebrain neurons. All FXG types associate with ribosomes and mRNA, but the specific mRNA cargos are a function of FXG type, brain region and neuron class. Transcripts for β‐catenin and its regulator APC associate with a subset of forebrain FXGs. Moreover, both these transcripts can colocalize within individual FXGs, suggesting that the axonal translation of functionally related proteins may be coordinately regulated with high spatiotemporal resolution. Cell type‐dependent expression of specific RNP types with distinct mRNA cargos, such as FXGs, presents a potential mechanism for regulating local translation and its output in a circuit‐dependent manner.
Fragile X granules (FXGs) are axonal mRNA granules that contain one or more of the Fragile X related (FXR) family of translational regulator proteins. Combined immunofluorescence and in situ hybridization revealed that FXGs exhibit circuit dependence in both the FXR proteins they contain and the mRNAs with which they associate.
Metrics
Details
- Title
- Fragile X granules are a family of axonal ribonucleoprotein particles with circuit‐dependent protein composition and mRNA cargos
- Creators
- Eunice Chyung - Brown UniversityHannah F. LeBlanc - Brown UniversityJustin R. Fallon - Brown UniversityMichael R. Akins - Drexel University
- Publication Details
- Journal of comparative neurology (1911), v 526(1), pp 96-108
- Publisher
- Wiley
- Number of pages
- 13
- Grant note
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD052083) National Institute of Mental Health (MH090237)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000418575500006
- Scopus ID
- 2-s2.0-85030261017
- Other Identifier
- 991019168903004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences
- Zoology