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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Fully 3D printed integrated reactor array for point-of-care molecular diagnostics
Biosensors & bioelectronics, v 109, pp 156-163
30 Jun 2018
PMID: 29550739
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Molecular diagnostics that involve nucleic acid amplification tests (NAATs) are crucial for prevention and treatment of infectious diseases. In this study, we developed a simple, inexpensive, disposable, fully 3D printed microfluidic reactor array that is capable of carrying out extraction, concentration and isothermal amplification of nucleic acids in variety of body fluids. The method allows rapid molecular diagnostic tests for infectious diseases at point of care. A simple leak-proof polymerization strategy was developed to integrate flow-through nucleic acid isolation membranes into microfluidic devices, yielding a multifunctional diagnostic platform. Static coating technology was adopted to improve the biocompatibility of our 3D printed device. We demonstrated the suitability of our device for both end-point colorimetric qualitative detection and real-time fluorescence quantitative detection. We applied our diagnostic device to detection of Plasmodium falciparum in plasma samples and Neisseria meningitides in cerebrospinal fluid (CSF) samples by loop-mediated, isothermal amplification (LAMP) within 50 min. The detection limits were 100 fg for P. falciparum and 50 colony-forming unit (CFU) for N. meningitidis per reaction, which are comparable to that of benchtop instruments. This rapid and inexpensive 3D printed device has great potential for point-of-care molecular diagnosis of infectious disease in resource-limited settings.
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Details
- Title
- Fully 3D printed integrated reactor array for point-of-care molecular diagnostics
- Creators
- Karteek Kadimisetty - University of PennsylvaniaJinzhao Song - University of PennsylvaniaAoife M. Doto - University of PennsylvaniaYoung Hwang - College Station Medical CenterJing Peng - University of PennsylvaniaMichael G. Mauk - Univ Penn, Dept Mech Engn & Appl Mech, 210 Towne Bldg,220 South 33rd St, Philadelphia, PA 19104 USAFrederic D. Bushman - University of PennsylvaniaRobert Gross - University of PennsylvaniaJoseph N. Jarvis - University of PennsylvaniaChangchun Liu - University of Pennsylvania
- Publication Details
- Biosensors & bioelectronics, v 109, pp 156-163
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- AI045008; R01EB023607; R01CA214072; R21TW010625 / Penn Center for AIDS Research (Penn CFAR) Pilot Grant R01CA214072 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) P30AI045008 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) R01EB023607 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB) R21TW010625 / FOGARTY INTERNATIONAL CENTER; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Fogarty International Center (FIC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000430778400021
- Scopus ID
- 2-s2.0-85043581289
- Other Identifier
- 991020623909104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology