Journal article
Fusion-related Release of Glutamate from Astrocytes
The Journal of biological chemistry, v 279(13), pp 12724-12733
26 Mar 2004
PMID: 14722063
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Although cell culture studies have implicated the presence of vesicle proteins in mediating the release of glutamate from astrocytes, definitive proof requires the identification of the glutamate release mechanism and the localization of this mechanism in astrocytes at synaptic locales. In cultured murine astrocytes we show an array of vesicle proteins, including SNARE proteins, and vesicular glutamate transporters that are required to fill vesicles with glutamate. Using immunocytochemistry and single-cell multiplex reverse transcription-PCR we demonstrate the presence of these proteins and their transcripts within astrocytes freshly isolated from the hippocampus. Moreover, immunoelectron microscopy demonstrates the presence of VGLUT1 in processes of astrocytes of the hippocampus. To determine whether calcium-dependent glutamate release is mediated by exocytosis, we expressed the SNARE motif of synaptobrevin II to prevent the formation of SNARE complexes, which reduces glutamate release from astrocytes. To further determine whether vesicular exocytosis mediates calcium-dependent glutamate release from astrocytes, we performed whole cell capacitance measurements from individual astrocytes and demonstrate an increase in whole cell capacitance, coincident with glutamate release. Together, these data allow us to conclude that astrocytes in situ express vesicle proteins necessary for filling vesicles with the chemical transmitter glutamate and that astrocytes release glutamate through a vesicle- or fusion-related mechanism.
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Details
- Title
- Fusion-related Release of Glutamate from Astrocytes
- Creators
- Qi Zhang - University of PennsylvaniaTina Pangršič - Celica (Slovenia)Marko Kreft - University of LjubljanaMojca Kržan - University of LjubljanaNianzhen Li - University of WashingtonJai-Yoon Sul - University of PennsylvaniaMichael Halassa - University of PennsylvaniaElisabeth Van Bockstaele - Thomas Jefferson UniversityRobert Zorec - University of LjubljanaPhilip G. Haydon - University of Pennsylvania
- Publication Details
- The Journal of biological chemistry, v 279(13), pp 12724-12733
- Publisher
- Elsevier
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000220334900086
- Scopus ID
- 2-s2.0-11144358154
- Other Identifier
- 991021903301104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology